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On-line high-performance liquid affinity chromatography-high-performance liquid chromatography analysis of monomeric ribonucleoside compounds in biological fluids.

作者信息

Hagemeier E, Kemper K, Boos K S, Schlimme E

出版信息

J Chromatogr. 1983 Dec 30;282:663-9. doi: 10.1016/s0021-9673(00)91643-1.

DOI:10.1016/s0021-9673(00)91643-1
PMID:6674319
Abstract

We describe an on-line multi-dimensional column chromatography system. It consists of a high-performance liquid affinity chromatography perfractionation column for cis-diol compounds and a series-connected reversed-phase high-performance liquid chromatography system for the analysis of ribonucleosides. This on-line procedure allows the rapid and direct analysis of methylated ribonucleic acid catabolites in biological fluids (serum, urine) and therefore might be useful in pathobiochemistry.

摘要

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