School of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, Henan, 453003, People's Republic of China.
College of Medical Engineering, Xinxiang Medical University, Henan 453003, People's Republic of China.
Int J Nanomedicine. 2020 May 8;15:3281-3290. doi: 10.2147/IJN.S240300. eCollection 2020.
Cells exhibit high sensitivity and a diverse response to the nanotopography of the extracellular matrix, thereby endowing materials with instructive performances formerly reserved for growth factors. This finding leads to opportunities for improvement. However, the interplay between the topographical surface and cell behaviors remains incompletely understood.
In the present study, we showed nanosurfaces with various dimensions of nanopits (200-750 nm) fabricated by self-assembling polystyrene (PS) nanospheres. Human adipose-derived stem cell behaviors, such as cell morphology, adhesion, cytoskeleton contractility, proliferation, and differentiation, were investigated on the prepared PS nanopit surface.
The osteogenic differentiation can be enhanced by nanopits with a diameter of 300-400 nm.
The present study provided exciting new avenues to investigate cellular responses to well-defined nanoscale topographic features, which could further guide bone tissue engineering and stem cell clinical research. The capability to control developing biomaterials mimicking nanotopographic surfaces promoted functional tissue engineering, such as artificial joint replacement, bone repair, and dental applications.
细胞对外界基质的纳米形貌表现出高度的敏感性和多样化的反应，从而使材料具有以前只有生长因子才具备的指导性能。这一发现为改进提供了机会。然而，表面形貌与细胞行为之间的相互作用仍不完全清楚。
在本研究中，我们展示了通过自组装聚苯乙烯（PS）纳米球制备的具有不同尺寸纳米坑（200-750nm）的纳米表面。研究了人脂肪干细胞在制备的 PS 纳米坑表面上的行为，如细胞形态、黏附、细胞骨架收缩性、增殖和分化。
直径为 300-400nm 的纳米坑可增强成骨分化。
本研究为研究细胞对明确定义的纳米形貌特征的反应提供了令人兴奋的新途径，这可能进一步指导骨组织工程和干细胞临床研究。控制仿生纳米形貌表面的新型生物材料的能力促进了功能组织工程，如人工关节置换、骨修复和牙科应用。
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