Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-17165 Stockholm, Sweden.
Chem Commun (Camb). 2020 Jun 25;56(51):6989-6992. doi: 10.1039/d0cc00329h.
Fluorescence in vitro bioimaging suffers from photobleaching of organic dyes, thus, functional probes with superior photostability are urgently needed. Here, we address this challenge by developing novel silica-coated nanophosphors that may serve as superior luminescent nanoprobes compatible with conventional fluorescence microscopes. We specifically explore their suitability for dynamic in vitro bioimaging of interactions between bacterial pathogens and host cells, and further demonstrate the facile surface functionalization of the amorphous silica layer with antibodies for selective cell targeting.
体外荧光生物成像受到有机染料光漂白的限制，因此迫切需要具有优越光稳定性的功能探针。在这里，我们通过开发新型的硅涂层纳米荧光粉来应对这一挑战，它们可用作与传统荧光显微镜兼容的优越发光纳米探针。我们特别研究了它们在体外动态生物成像中用于研究细菌病原体与宿主细胞相互作用的适用性，进一步证明了通过简单的表面功能化在无定形硅层上接枝抗体，从而实现对细胞的选择性靶向。
