Kim DongHyeon, Yun JeaHyeok, Roh Eunmiri, Shin Han-Seung, Kim Jong-Eun
Department of Food Science and Biotechnology, Dongguk University-Seoul, Goyang-si 10326, Republic of Korea.
Department of Food Science and Technology, Korea National University of Transportation, Jeungpyeong 27909, Republic of Korea.
Plants (Basel). 2023 Jun 28;12(13):2479. doi: 10.3390/plants12132479.
Environmental pollutants such as fine dust are increasingly linked to premature skin aging. In this study, we investigated the protective effects of higenamine, a natural plant alkaloid, against fine-dust-induced skin aging in human keratinocytes (HaCaT cells). We found that higenamine significantly attenuated fine-dust-induced expression of matrix metalloproteinase-1 (MMP-1), a key enzyme involved in collagen degradation. Furthermore, higenamine was found to modulate fine-dust-induced AP-1 and NF-κB transactivation, which are crucial factors for MMP-1 transcription. Higenamine also impeded fine-dust-induced phosphorylation in specific pathways related to AP-1 and NF-κB activation, and effectively alleviated reactive oxygen species (ROS) production, a key factor in oxidative stress caused by fine dust exposure. These results suggest that higenamine exerts protective effects against fine-dust-induced skin aging, primarily through its MMP-1 inhibitory properties and ability to mitigate ROS-induced oxidative damage. Our data highlight the potential of higenamine as an effective ingredient in skincare products designed to combat environmental skin damage.
细粉尘等环境污染物与皮肤过早老化的关联日益密切。在本研究中，我们调查了天然植物生物碱去甲乌药碱对细粉尘诱导的人角质形成细胞（HaCaT细胞）皮肤老化的保护作用。我们发现，去甲乌药碱显著减弱了细粉尘诱导的基质金属蛋白酶-1（MMP-1）的表达，MMP-1是参与胶原蛋白降解的关键酶。此外，发现去甲乌药碱可调节细粉尘诱导的AP-1和NF-κB反式激活，这是MMP-1转录的关键因素。去甲乌药碱还阻碍了与AP-1和NF-κB激活相关的特定途径中细粉尘诱导的磷酸化，并有效减轻了活性氧（ROS）的产生，ROS是细粉尘暴露引起的氧化应激的关键因素。这些结果表明，去甲乌药碱主要通过其MMP-1抑制特性和减轻ROS诱导的氧化损伤的能力，对细粉尘诱导的皮肤老化发挥保护作用。我们的数据凸显了去甲乌药碱作为一种有效成分用于设计对抗环境性皮肤损伤的护肤品的潜力。
