植物乳杆菌 12 胞外多糖对福氏志贺菌的抗生物膜活性。
Antibiofilm Activity of Lactobacillus plantarum 12 Exopolysaccharides against Shigella flexneri.
机构信息
School of Food Science and Technology, Dalian Polytechnic University, Dalian, People's Republic of China.
School of Food Science and Technology, Dalian Polytechnic University, Dalian, People's Republic of China
出版信息
Appl Environ Microbiol. 2020 Jul 20;86(15). doi: 10.1128/AEM.00694-20.
In developing countries, is the most common enteric pathogen causing bacillary dysentery. Biofilm formation by can cause the emergence of antibiotic-resistant strains, which poses serious threats to food safety and human health. In this study, the effects of 12 exopolysaccharides (L-EPSs) and exopolysaccharides (S-EPSs) on CMCC51574 biofilm formation were investigated. The results showed that L-EPS could decrease polysaccharide production in the extracellular polymeric matrix of and inhibit biofilm formation by L-EPS could decrease the minimum biofilm elimination concentration (MBEC) of antibiotics against biofilm and inhibit adhesion to and invasion into HT-29 cell monolayers, which might be ascribed to biofilm disturbance by L-EPS. In contrast, S-EPS exhibited the opposite effects compared to L-EPS. The monosaccharide composition analysis showed that L-EPS was composed of mannose, glucuronic acid, galactosamine, glucose, galactose, and xylose, with the molar ratio of 32.26:0.99:1.79:5.63:0.05:4.07, while S-EPS was composed of mannose, glucuronic acid, galactosamine, glucose, and galactose, with the molar ratio of 25.43:2.28:7.13:5.35. L-EPS was separated into the neutral polysaccharide L-EPS 1-1 and the acidic polysaccharide L-EPS 2-1 by ion-exchange chromatography and gel chromatography. L-EPS 2-1 exerted higher antibiofilm activity than L-EPS 1-1. The antibiofilm activity of L-EPS might be associated with its structure. is a widespread foodborne pathogen causing food contamination and responsible for food poisoning outbreaks related to various foods in developing countries. Not only has biofilm formation by been difficult to eliminate, but it has also increased the drug resistance of the strain. In the present study, it was demonstrated that L-EPSs secreted by 12 could inhibit biofilm formation on, adhesion to, and invasion into HT-29 cells. Also, L-EPSs could decrease the minimum biofilm elimination concentration (MBEC) of the antibiotics used against biofilm. Therefore, L-EPSs were shown to be bioactive macromolecules with the potential ability to act against infections.
在发展中国家，是引起细菌性痢疾的最常见肠道病原体。生物膜的形成可导致抗生素耐药菌株的出现，对食品安全和人类健康构成严重威胁。在本研究中，研究了 12 种胞外多糖（L-EPSs）和 种胞外多糖（S-EPSs）对 CMCC51574 生物膜形成的影响。结果表明，L-EPS 可减少 胞外聚合基质中多糖的产生，抑制生物膜的形成，L-EPS 可降低抗生素对生物膜的最低抑菌浓度（MBEC），并抑制 对 HT-29 细胞单层的黏附和侵袭，这可能归因于 L-EPS 对生物膜的干扰。相比之下，S-EPS 与 L-EPS 的作用相反。单糖组成分析表明，L-EPS 由甘露糖、葡萄糖醛酸、半乳糖胺、葡萄糖、半乳糖和木糖组成，摩尔比为 32.26:0.99:1.79:5.63:0.05:4.07，而 S-EPS 由甘露糖、葡萄糖醛酸、半乳糖胺、葡萄糖和半乳糖组成，摩尔比为 25.43:2.28:7.13:5.35。L-EPS 通过离子交换层析和凝胶层析分离为中性多糖 L-EPS 1-1 和酸性多糖 L-EPS 2-1。L-EPS 2-1 比 L-EPS 1-1 具有更高的抗生物膜活性。L-EPS 的抗生物膜活性可能与其结构有关。是一种广泛存在的食源性病原体，可导致食物污染，并导致发展中国家与各种食物有关的食物中毒暴发。不仅难以消除 的生物膜形成，而且还增加了菌株的耐药性。在本研究中，证明了 12 分泌的 L-EPS 可抑制生物膜在 上的形成、黏附和侵袭 HT-29 细胞。此外，L-EPS 还可降低用于对抗 生物膜的抗生素的最低生物膜消除浓度（MBEC）。因此，L-EPS 是具有抗 感染潜力的生物活性大分子。
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