Department of Biological Science and Technology, National Pingtung University of Science and Technology.
Department of Biological Science and Technology, Meiho University; Department of Nursing, Meiho University.
Int J Biol Macromol. 2019 Jul 15;133:575-582. doi: 10.1016/j.ijbiomac.2019.04.109. Epub 2019 Apr 18.
Exopolysaccharides (EPSs) are metabolites of probiotics that have gained wide interest recently. A strain of Lactobacillus reuteri Mh-001 with high exopolysaccharide (EPS) production ability was isolated, identified, and were used to investigate the anti-inflammatory effects of the EPSs. Among the three unpurified EPSs, RAW246.7 murine macrophages treated with 5 ppm of EPS 1 revealed the lowest tumour necrosis factor α (TNF-α) secretion (325.32 ± 51.10 pg/ug DNA). The second lowest TNF- α secretion occurred with EPS 2 (701.12 ± 86.108 pg/ug DNA) from Mh-002. EPSs 4, 5, and 6 were further purified from EPS 1. Cells treated with 1 ppm of EPS 4 had the lowest TNF-α secretion of all (209.20 ± 84.34 pg/ug DNA). The monosaccharide components, EPS 4 and EPS 1, had the highest galactose content (45 ± 2.75% and 39 ± 2.75%, respectively). The monosaccharide percentages (galactose > rhamnose > glucose) were related to the anti-inflammatory activity of the EPSs. The galactose content of EPSs enhanced their anti-inflammatory effects on the macrophages. These data indicate that EPS possesses beneficial physiological effects such as anti-inflammatory properties, and the monosaccharide content of the EPS was the factor influencing the anti-inflammatory properties.
胞外多糖（EPSs）是益生菌的代谢产物，最近引起了广泛关注。本研究从分离的具有高产胞外多糖（EPS）能力的罗伊氏乳杆菌 Mh-001 中鉴定出一种菌株，并研究了 EPS 的抗炎作用。在三种未纯化的 EPS 中，RAW246.7 鼠巨噬细胞用 5ppm 的 EPS1 处理后，肿瘤坏死因子-α（TNF-α）的分泌量最低（325.32±51.10pg/ug DNA）。其次是 EPS2（701.12±86.108pg/ug DNA），它来自 Mh-002。从 EPS1 进一步纯化了 EPS4、EPS5 和 EPS6。用 1ppm 的 EPS4 处理细胞后，TNF-α的分泌量最低（209.20±84.34pg/ug DNA）。单糖成分 EPS4 和 EPS1 的半乳糖含量最高（分别为 45±2.75%和 39±2.75%）。单糖百分比（半乳糖>鼠李糖>葡萄糖）与 EPS 的抗炎活性有关。EPS 中的半乳糖含量增强了其对巨噬细胞的抗炎作用。这些数据表明 EPS 具有有益的生理作用，如抗炎特性，并且 EPS 的单糖含量是影响抗炎特性的因素。
