Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China.
Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China; Beijing Key Laboratory of Radioactive Wastes Treatment, Tsinghua University, Beijing 100084, PR China.
Bioresour Technol. 2018 Jan;247:954-962. doi: 10.1016/j.biortech.2017.09.041. Epub 2017 Sep 8.
In this study, five pretreatment methods (heat shock, acid, base, aeration and gamma radiation) were applied for enriching hydrogen producers from anaerobically digested sludge, aiming to compare their hydrogen fermentation performance using raw ryegrass as substrate. Results showed that various pretreatment methods caused great variations on grass hydrogen fermentation performance. Acid pretreatment was most efficient compared with other tested pretreatment methods, with relevant hydrogen yield of 64.4mL/g dry grass and organics removal of 31.4%. Kinetics results showed that the first-order kinetic model fitted hydrogen evolution better than the modified Gompertz model. Microbiological analysis showed that various pretreatment methods caused great variations on microbial activity and microbial community composition. Clostridium and Enterococcus were two dominant genera, while relative abundances of these two genera varied greatly for different pretreated samples. Difference in microbial activity and microbial community distribution induced by the pretreatment methods might directly cause different ryegrass fermentation performance.
在这项研究中，应用了五种预处理方法（热冲击、酸、碱、曝气和伽马辐射）来富集从厌氧消化污泥中产生氢气的产氢菌，旨在比较使用新鲜黑麦草作为底物时它们的氢气发酵性能。结果表明，各种预处理方法对黑麦草的氢气发酵性能有很大的影响。与其他测试的预处理方法相比，酸预处理是最有效的，相关的氢气产率为 64.4 毫升/克干草，有机物去除率为 31.4%。动力学结果表明，一级动力学模型比修正的 Gompertz 模型更适合描述氢气的产生。微生物分析表明，各种预处理方法对微生物活性和微生物群落组成有很大的影响。梭菌属和肠球菌属是两个主要的属，而这两个属的相对丰度在不同的预处理样品中差异很大。预处理方法引起的微生物活性和微生物群落分布的差异可能直接导致黑麦草发酵性能的不同。
