Department of Chemistry "Ugo Schiff" and CSGI, University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
Department of Analytical Chemistry and Chemical Metallurgy, Wroclaw University of Science and Technology, Faculty of Chemistry, 27 Wybrzeze Wyspianskiego, 50-370 Wroclaw, Poland.
ACS Biomater Sci Eng. 2023 Dec 11;9(12):6632-6643. doi: 10.1021/acsbiomaterials.3c00817. Epub 2023 Nov 20.
Atmospheric pressure plasma treatments are nowadays gaining importance to improve the performance of biomaterials in the orthopedic field. Among those, magnesium phosphate-based cements (MPCs) have recently shown attractive features as bone repair materials. The effect of plasma treatments on such cements, which has not been investigated so far, could represent an innovative strategy to modify MPCs' physicochemical properties and to tune their interaction with cells. MPCs were prepared and treated for 5, 7.5, and 10 min with a cold atmospheric pressure plasma jet. The reactive nitrogen and oxygen species formed during the treatment were characterized. The surfaces of MPCs were studied in terms of the phase composition, morphology, and topography. After a preliminary test in simulated body fluid, the proliferation, adhesion, and osteogenic differentiation of human mesenchymal cells on MPCs were assessed. Plasma treatments induce modifications in the relative amounts of struvite, newberyite, and farringtonite on the surfaces on MPCs in a time-dependent fashion. Nonetheless, all investigated scaffolds show a good biocompatibility and cell adhesion, also supporting osteogenic differentiation of mesenchymal cells.
大气压等离子体处理在改善骨科领域生物材料性能方面的重要性日益增加。其中，基于磷酸镁的水泥（MPC）最近作为骨修复材料显示出了有吸引力的特性。迄今为止，尚未研究过等离子体处理对这些水泥的影响，这可能代表着一种改进 MPC 物理化学性质并调整其与细胞相互作用的创新策略。将 MPC 制备并分别用冷大气压等离子射流处理 5、7.5 和 10 分钟。对处理过程中形成的反应性氮和氧物种进行了表征。从相组成、形貌和形貌方面研究了 MPC 的表面。在模拟体液的初步测试后，评估了人骨髓间充质细胞在 MPC 上的增殖、黏附和成骨分化。等离子体处理以时间依赖性的方式在 MPC 表面诱导鸟粪石、新伯雷石和法林顿石的相对量发生变化。尽管如此，所有研究的支架都表现出良好的生物相容性和细胞黏附性，也支持间充质细胞的成骨分化。
