Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, Japan.
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
J Biosci Bioeng. 2020 Sep;130(3):253-259. doi: 10.1016/j.jbiosc.2020.04.010. Epub 2020 May 22.
Ribozymes with modular structures are attractive platforms for the construction of nanoscale RNA objects with biological functions. We designed group I ribozyme dimers as unit ribozyme dimers (Urds), which self-assembled to form their polymeric states and also oligomeric states with defined numbers of Urds. Assembly of Urds yielded catalytic ability of a pair of distinct ribozyme units to cleave two distinct substrates. The morphologies of the assembled ribozyme structures were observed directly by atomic force microscopy (AFM).
具有模块化结构的核酶是构建具有生物功能的纳米级 RNA 结构的理想平台。我们设计了 I 组核酶二聚体作为单元核酶二聚体 (Urd)，它们可以自我组装形成多聚体状态，也可以形成具有特定 Urd 数量的寡聚体状态。Urd 的组装产生了一对不同核酶单元切割两个不同底物的催化能力。组装核酶结构的形态可以通过原子力显微镜 (AFM) 直接观察到。
