Mori Yuki, Oi Hiroki, Suzuki Yuki, Hidaka Kumi, Sugiyama Hiroshi, Endo Masayuki, Matsumura Shigeyoshi, Ikawa Yoshiya
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, 930-8555, Toyama, Japan.
Department of Chemistry, Graduate School of Science, Kyoto University, 606-8502, Kyoto, Japan.
Chembiochem. 2021 Jun 15;22(12):2168-2176. doi: 10.1002/cbic.202100109. Epub 2021 May 6.
Ribozymes with modular architecture constitute an attractive class of structural platforms for design and construction of nucleic acid nanostructures with biological functions. Through modular engineering of the Tetrahymena ribozyme, we have designed unit RNAs (L-RNAs), assembly of which formed ribozyme-based closed trimers and closed tetramers. Their catalytic activity was dependent on oligomer formation. In this study, the structural variety of L-RNA oligomers was extended by tuning their structural elements, yielding closed pentamers and closed hexamers. Their assembly properties were analyzed by electrophoretic mobility shift assay (EMSA) and atomic force microscopy (AFM).
具有模块化结构的核酶构成了一类极具吸引力的结构平台，可用于设计和构建具有生物学功能的核酸纳米结构。通过对嗜热四膜虫核酶进行模块化工程改造，我们设计了单元RNA（L-RNA），其组装形成了基于核酶的封闭三聚体和封闭四聚体。它们的催化活性取决于寡聚体的形成。在本研究中，通过调整L-RNA寡聚体的结构元件，扩展了其结构多样性，得到了封闭五聚体和封闭六聚体。通过电泳迁移率变动分析（EMSA）和原子力显微镜（AFM）对它们的组装特性进行了分析。
