曼氏血吸虫磷酸丙糖异构酶的晶体学研究。

Crystallographic Studies of Triosephosphate Isomerase from Schistosoma mansoni.

机构信息

Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.

Langebio-Cinvestav Sede Irapuato, Guanajuato, Mexico.

出版信息

Methods Mol Biol. 2020;2151:211-218. doi: 10.1007/978-1-0716-0635-3_17.

DOI:10.1007/978-1-0716-0635-3_17

PMID:32452007

Abstract

Protein structure determination by X-ray crystallography guides structure-function and rational drug design studies. Helminths cause devastating diseases, including schistosomiasis that affects over one-third of the human population. Trematodes from the genus Schistosoma heavily depend on glycolysis; thus enzymes involved in this metabolic pathway are potential drug targets. Here we present a protocol to obtain crystal structures of recombinantly expressed triosephosphate isomerase from S. mansoni (SmTPI) that diffracted in house to a resolution of 2 Å.

摘要

通过 X 射线晶体学确定蛋白质结构可指导结构-功能和合理药物设计研究。寄生虫会导致严重的疾病，包括影响超过三分之一人口的血吸虫病。曼森血吸虫属的吸虫严重依赖糖酵解；因此，参与这种代谢途径的酶是潜在的药物靶点。在这里，我们提供了一个从曼氏血吸虫（SmTPI）中获得重组表达的磷酸丙糖异构酶（SmTPI）晶体结构的方案，该晶体结构在室内衍射至 2Å 的分辨率。