精油对HT-29和HeLa细胞系细胞毒性的实时细胞分析
Real-Time Cell Analysis of the Cytotoxicity of Essential Oil on HT-29 and HeLa Cell Lines.
作者信息
Öke Altuntaş Feyza, Demirtaş İbrahim
机构信息
Gazi University, Faculty Of Science, Department Of Biology, Ankara, Turkey.
Çankırı Karatekin University, Faculty of Science, Department of Chemistry, Çankırı, Turkey.
出版信息
Turk J Pharm Sci. 2017 Apr;14(1):29-33. doi: 10.4274/tjps.40427. Epub 2017 Apr 15.
OBJECTIVES
The aim of this study was to investigate the cytotoxic and radical scavenging effects of the essential oil from (Hand-Mazz.) Ietswaart and to determine its chemical composition.
MATERIALS AND METHODS
The essential oil was obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC-MS). The cytotoxic effect of the essential oil on the growth of human colorectal adenocarcinoma (HT-29) and human cervical adenocarcinoma (HeLa) cell lines was investigated by xCELLigence Real Time Cell Analyzer instrument. Moreover, radical scavenging effect of the oil was determined by using DPPH method.
RESULTS
The main constituents of the oil were found to be carvacrol (61.69%), -cymene (17.32%), γ-terpinene (4.05%), and borneol (3.96%). The essential oil of exhibited significant cytotoxic effect against HeLa and HT-29 cell lines at the tested concentrations. The essential oil had moderate DPPH radical scavenging activity compared to butylated hydroxytoluene (BHT).
CONCLUSION
The data in the present study clearly demonstrated inhibitory effect of the oil on two human cancer cell lines. According to these results and other reported studies, this observed high effect may be attributed to the presence of the carvacrol component in the oil.
目的
本研究旨在探究（Hand-Mazz.）Ietswaart精油的细胞毒性和自由基清除作用，并确定其化学成分。
材料与方法
通过水蒸馏法获得精油，并采用气相色谱/质谱联用仪（GC-MS）进行分析。使用xCELLigence实时细胞分析仪研究该精油对人结肠腺癌（HT-29）和人宫颈腺癌（HeLa）细胞系生长的细胞毒性作用。此外，采用DPPH法测定该精油的自由基清除作用。
结果
发现该精油的主要成分是香芹酚（61.69%）、对异丙基甲苯（17.32%）、γ-萜品烯（4.05%）和冰片（3.96%））。在测试浓度下，该精油对HeLa和HT-29细胞系表现出显著的细胞毒性作用。与丁基羟基甲苯（BHT）相比，该精油具有中等程度的DPPH自由基清除活性。
结论
本研究中的数据清楚地表明了该精油对两种人类癌细胞系的抑制作用。根据这些结果和其他已报道的研究，观察到的这种高活性可能归因于该精油中香芹酚成分的存在。
Zotero 插件