Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil.
Graduate Program of Physical Education, Universidade Católica de Brasília, Distrito Federal, Brazil.
Oxid Med Cell Longev. 2020 May 4;2020:5603580. doi: 10.1155/2020/5603580. eCollection 2020.
Ancestral obesogenic exposure is able to trigger harmful effects in the offspring left ventricle (LV) which could lead to cardiovascular diseases. However, the impact of the father's lifestyle on the offspring LV is largely unexplored. The aim of this study was to investigate the effects of 8 weeks of paternal resistance training (RT) on the offspring left ventricle (LV) proteome exposed to control or high-fat (HF) diet. Wistar rats were randomly divided into two groups: sedentary fathers and trained fathers (8 weeks, 3 times per week with weights secured to the animals' tails). The offspring were obtained by mating with sedentary females. Upon weaning, male offspring were divided into 4 groups (5 animals per group): offspring from sedentary fathers, exposed to control diet (SFO-C); offspring from trained fathers, exposed to control diet (TFO-C); offspring from sedentary fathers, exposed to high-fat diet (SFO-HF); and offspring from trained fathers, exposed to high-fat diet (TFO-HF). The LC-MS/MS analysis revealed 537 regulated proteins among groups. Offspring exposure to HF diet caused reduction in the abundance levels of proteins related to cell component organization, metabolic processes, and transport. Proteins related to antioxidant activity, transport, and transcription regulation were increased in TFO-C and TFO-HF as compared with the SFO-C and SFO-HF groups. Paternal RT demonstrated to be an important intervention capable of inducing significant effects on the LV proteome regardless of offspring diet due to the increase of proteins involved into LV homeostasis maintenance. This study contributes to a better understanding of the molecular aspects involved in transgenerational inheritance.
祖先肥胖相关暴露能够在后代左心室 (LV) 中引发有害影响，从而导致心血管疾病。然而，父亲的生活方式对后代 LV 的影响在很大程度上尚未得到探索。本研究旨在研究 8 周的父亲阻力训练 (RT) 对暴露于对照或高脂肪 (HF) 饮食的后代左心室 (LV) 蛋白质组的影响。Wistar 大鼠被随机分为两组：久坐不动的父亲和训练有素的父亲（每周 3 次，尾巴上绑有重物）。通过与久坐不动的雌性交配获得后代。断奶后，雄性后代被分为 4 组（每组 5 只动物）：来自久坐不动的父亲、暴露于对照饮食的后代 (SFO-C)；来自训练有素的父亲、暴露于对照饮食的后代 (TFO-C)；来自久坐不动的父亲、暴露于高脂肪饮食的后代 (SFO-HF)；以及来自训练有素的父亲、暴露于高脂肪饮食的后代 (TFO-HF)。LC-MS/MS 分析显示各组之间有 537 个调节蛋白。后代暴露于 HF 饮食导致与细胞成分组织、代谢过程和运输相关的蛋白质丰度水平降低。与 SFO-C 和 SFO-HF 组相比，TFO-C 和 TFO-HF 组中与抗氧化活性、运输和转录调节相关的蛋白质增加。与对照饮食相比，父亲的 RT 表现为一种重要的干预措施，能够在 LV 蛋白质组中引起显著的影响，无论后代饮食如何，这是由于参与 LV 动态平衡维持的蛋白质增加。这项研究有助于更好地理解跨代遗传中涉及的分子方面。
