Pandey Tripti, Ghosh Arpita, Todur Vivek N, Rajendran Vijayakumar, Kalita Parismita, Kalita Jupitara, Shukla Rohit, Chetri Purna B, Shukla Harish, Sonkar Amit, Lyngdoh Denzelle Lee, Singh Radhika, Khan Heena, Nongkhlaw Joplin, Das Kanhu Charan, Tripathi Timir
Molecular and Structural Biophysics Laboratory, Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
Eurofins Genomics India Pvt. Ltd., Doddanekkundi, Bengaluru 560048, India.
ACS Omega. 2020 May 6;5(19):11084-11091. doi: 10.1021/acsomega.0c00980. eCollection 2020 May 19.
Fascioliasis, a neglected foodborne disease caused by liver flukes (genus ), affects more than 200 million people worldwide. Despite technological advances, little is known about the molecular biology and biochemistry of these flukes. We present the draft genome of for the first time. The assembled draft genome has a size of ∼1.04 Gb with an N50 and N90 of 129 and 149 kb, respectively. A total of 20 858 genes were predicted. The repeats identified in the draft genome were 46.85%. The pathway included all of the genes of glycolysis, Krebs cycle, and fatty acid metabolism but lacked the key genes of the fatty acid biosynthesis pathway. This indicates that the fatty acid required for survival of the fluke may be acquired from the host bile. It may be hypothesized that the relatively larger genome did not evolve through genome duplications but rather is interspersed with many repetitive elements. The genomic information will provide a comprehensive resource to facilitate the development of novel interventions for fascioliasis control.
肝片吸虫病是一种由肝吸虫（属）引起的被忽视的食源性疾病，全球有超过2亿人受其影响。尽管技术不断进步，但对这些吸虫的分子生物学和生物化学仍知之甚少。我们首次公布了[吸虫名称]的基因组草图。组装后的基因组草图大小约为1.04 Gb，N50和N90分别为129 kb和149 kb。共预测到20858个基因。基因组草图中鉴定出的重复序列占46.85%。该途径包括糖酵解、三羧酸循环和脂肪酸代谢的所有基因，但缺乏脂肪酸生物合成途径的关键基因。这表明吸虫生存所需的脂肪酸可能从宿主胆汁中获取。可以推测，相对较大的[吸虫名称]基因组并非通过基因组复制进化而来，而是散布着许多重复元件。这些基因组信息将提供全面的资源，以促进开发控制肝片吸虫病的新干预措施。
