Phillippy B Q, Bland J M
Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70124.
Anal Biochem. 1988 Nov 15;175(1):162-6. doi: 10.1016/0003-2697(88)90374-0.
Inositol phosphates including phytic acid were separated in 30 min by gradient ion chromatography with postcolumn derivatization. All four pentakisphosphates were resolved, while four tetrakisphosphate peaks were detected. The limits of detection for all polyphosphates, including tris- and bisphosphates, were between 1 and 2 nmol. The method was used to compare nonenzymatic dephosphorylation of inositol hexakisphosphate at pH 4.0 versus pH 10.8. The only pentakisphosphate detected in calf brains was identified as myo-inositol 1,3,4,5,6-pentakisphosphate. The major pentakisphosphate in raw soybean seeds was myo-inositol 1,2,4,5,6-pentakisphosphate of unknown enantiomeric composition, while lesser amounts of myo-inositol 1,2,3,4,5-pentakisphosphate of unknown enantiomeric composition, myo-inositol 1,2,3,4,6-pentakisphosphate, and myo-inositol 1,3,4,5,6-pentakisphosphate were also present.
通过柱后衍生梯度离子色谱法，在30分钟内分离出包括植酸在内的肌醇磷酸酯。所有四种五磷酸酯都得到了分离，同时检测到四个四磷酸酯峰。包括三磷酸酯和二磷酸酯在内的所有多磷酸酯的检测限在1至2纳摩尔之间。该方法用于比较肌醇六磷酸在pH 4.0和pH 10.8时的非酶促去磷酸化。在小牛大脑中检测到的唯一五磷酸酯被鉴定为肌醇1,3,4,5,6 - 五磷酸酯。生大豆种子中的主要五磷酸酯是对映体组成未知的肌醇1,2,4,5,6 - 五磷酸酯，同时还存在少量对映体组成未知的肌醇1,2,3,4,5 - 五磷酸酯、肌醇1,2,3,4,6 - 五磷酸酯和肌醇1,3,4,5,6 - 五磷酸酯。
