由受体激活产生的肌醇磷酸合成肌醇1,3,4,5,6 - 五磷酸。
Synthesis of myo-inositol 1,3,4,5,6-pentakisphosphate from inositol phosphates generated by receptor activation.
作者信息
Stephens L R, Hawkins P T, Barker C J, Downes C P
机构信息
Smith Kline & French Research Ltd., Welwyn, Herts, U.K.
出版信息
Biochem J. 1988 Aug 1;253(3):721-33. doi: 10.1042/bj2530721.
myo-[3H]Inositol 1,3,4,5,6-pentakisphosphate can be made from myo-[3H]inositol 1,4,5-trisphosphate in a rat brain homogenate or soluble fraction. Although D-myo-inositol 3,4,5,6-tetrakisphosphate can be phosphorylated by a soluble rat brain enzyme to give myo-inositol 1,3,4,5,6-pentakisphosphate, it is not an intermediate in the pathway from myo-inositol 1,4,5-trisphosphate. The intermediates in the above pathway are myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 1,3,4-trisphosphate and myo-inositol 1,3,4,6-tetrakisphosphate [Shears, Parry, Tang, Irvine, Michell & Kirk (1987) Biochem. J. 246, 139-147; Balla, Guillemette, Baukal & Catt (1987) J. Biol. Chem. 262, 9952-9955], and it is catalysed by soluble kinase activities of similar anion-exchange mobility and Mr value. Compounds with chromatographic and chemical properties consistent with the structures myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 1,3,4,6-tetrakisphosphate and myo-inositol 3,4,5,6-tetrakisphosphate are present in avian erythrocytes, human 1321 N1 astrocytoma cells and primary-cultured murine bone-marrow-derived macrophages. The amounts of these inositol tetrakisphosphates rise upon muscarinic cholinergic stimulation of the astrocytoma cells or stimulation of macrophages with platelet-activating factor.
肌醇-[³H]1,3,4,5,6-五磷酸可由肌醇-[³H]1,4,5-三磷酸在大鼠脑匀浆或可溶性组分中合成。虽然D-肌醇3,4,5,6-四磷酸可被大鼠脑可溶性酶磷酸化生成肌醇1,3,4,5,6-五磷酸，但它不是肌醇1,4,5-三磷酸代谢途径的中间产物。上述途径的中间产物是肌醇1,3,4,5-四磷酸、肌醇1,3,4-三磷酸和肌醇1,3,4,6-四磷酸[希尔斯、帕里、唐、欧文、米切尔和柯克（1987年）《生物化学杂志》246卷，第139 - 147页；巴拉、吉耶梅特、鲍卡尔和卡特（1987年）《生物化学杂志》262卷，第9952 - 9955页]，且该过程由具有相似阴离子交换迁移率和相对分子质量的可溶性激酶活性催化。具有与肌醇1,3,4,5-四磷酸、肌醇1,3,4,6-四磷酸和肌醇3,4,5,6-四磷酸结构相符的色谱和化学性质的化合物存在于禽红细胞、人1321 N1星形细胞瘤细胞和原代培养的小鼠骨髓来源巨噬细胞中。这些肌醇四磷酸的量在星形细胞瘤细胞受到毒蕈碱胆碱能刺激或巨噬细胞受到血小板活化因子刺激时会增加。
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