College of Life Science, Sichuan Normal University, Sichuan, China.
Shengshi Rongen Bio-tech Co., Ltd., Shanghai, China.
J Med Food. 2020 Jul;23(7):772-782. doi: 10.1089/jmf.2019.4554. Epub 2020 May 26.
Honey bee larva powder (HLP) has traditionally been used as a daily supplement and tonic for health promotion with an uncertain scientific basis. In this study, B16-F10 tumor-bearing mice were established to evaluate the immunomodulatory activity of HLP. The proliferation and apoptosis assays were performed to evaluate the anti-inflammatory activity of honey bee larva extract (HLE) in RAW 264.7 macrophage. The experimental results demonstrated that the oral administration of freeze-dried HLP (4 and 6 g/kg) significantly enhanced the spleen index, the percentage of CD4cells, and the ratio of CD4 and CD8 T lymphocytes (CD4/CD8) in the peripheral blood compared with those in the tumor control mice. The studies demonstrated the potent immunomodulatory activities of HLE through the induction of RAW 264.7 macrophage proliferation and the mitigation of doxorubicin (DOX)-induced toxicity. HLE also exhibits anti-inflammatory activity by decreasing the production of nitric oxide (NO) and the cytokine level of interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage. The present study provides important scientific evidence for the immunomodulatory and anti-inflammatory activities of HLP and HLE.
蜂幼虫粉（HLP）传统上被用作日常补充剂和滋补品，以促进健康，但科学依据不确定。本研究建立了 B16-F10 荷瘤小鼠模型，以评估 HLP 的免疫调节活性。通过 RAW 264.7 巨噬细胞增殖和凋亡试验评估蜂蜜幼虫提取物（HLE）的抗炎活性。实验结果表明，与肿瘤对照组相比，口服冻干 HLP（4 和 6 g/kg）可显著提高脾脏指数、外周血中 CD4 细胞的百分比以及 CD4 和 CD8 T 淋巴细胞的比值（CD4/CD8）。研究表明，HLE 通过诱导 RAW 264.7 巨噬细胞增殖和减轻多柔比星（DOX）诱导的毒性，发挥强大的免疫调节活性。HLE 通过降低脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞中一氧化氮（NO）和白细胞介素-6（IL-6）细胞因子水平，表现出抗炎活性。本研究为 HLP 和 HLE 的免疫调节和抗炎活性提供了重要的科学证据。
