一个光依赖型类病变突变体的遗传定位揭示了大豆中粪卟啉原III氧化酶同源物的功能。
Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean.
作者信息
Ma Jingjing, Yang Suxin, Wang Dongmei, Tang Kuanqiang, Feng Xing Xing, Feng Xian Zhong
机构信息
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China.
University of Chinese Academy of Sciences, Beijing, China.
出版信息
Front Plant Sci. 2020 May 8;11:557. doi: 10.3389/fpls.2020.00557. eCollection 2020.
Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. Here, we isolated a (), which displayed a light-dependent cell death phenotype. Map-based cloning revealed that encods a coproporphyrinogen III oxidase and participates in tetrapyrrole biosynthesis. Knockout of led to necrotic spots on developing leaves of CRISPR/Cas9 induced mutants. The defect decreased the chlorophyll content by disrupting tetrapyrrole biosynthesis and enhanced resistance to . These results suggested that gene played an important role in the biosynthesis of tetrapyrrole and light-dependent defense in soybeans.
类病变模拟突变体为阐明细胞死亡和抗病性的分子机制提供了理想的遗传材料。在此，我们分离出了一个（），其表现出光依赖型细胞死亡表型。图位克隆显示，（）编码一种原卟啉原III氧化酶，并参与四吡咯生物合成。对（）的敲除导致CRISPR/Cas9诱导突变体的发育叶片上出现坏死斑点。（）缺陷通过破坏四吡咯生物合成降低了叶绿素含量，并增强了对（）的抗性。这些结果表明，（）基因在大豆四吡咯生物合成和光依赖型防御中发挥了重要作用。
Zotero 插件