Yao Dan, Zhou Junming, Zhang Aijing, Wang Jiaxin, Liu Yixuan, Wang Lixue, Pi Wenxuan, Li Zihao, Yue Wenjun, Cai Jinliang, Liu Huijing, Hao Wenyuan, Qu Xiangchun
College of Life Science, Jilin Agricultural University, Changchun, Jilin, China.
Institute of Crop Resources, Jilin Provincial Academy of Agricultural Sciences, Gongzhuling, Jilin, China.
Front Plant Sci. 2023 Aug 30;14:1247707. doi: 10.3389/fpls.2023.1247707. eCollection 2023.
Soybean [ (Linn.) Merr] is a source of plant-based proteins and an essential oilseed crop and industrial raw material. The increase in the demand for soybeans due to societal changes has coincided with the increase in the breeding of soybean varieties with enhanced traits. Earlier gene editing technologies involved zinc finger nucleases and transcription activator-like effector nucleases, but the third-generation gene editing technology uses clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The rapid development of CRISPR/Cas9 technology has made it one of the most effective, straightforward, affordable, and user-friendly technologies for targeted gene editing. This review summarizes the application of CRISPR/Cas9 technology in soybean molecular breeding. More specifically, it provides an overview of the genes that have been targeted, the type of editing that occurs, the mechanism of action, and the efficiency of gene editing. Furthermore, suggestions for enhancing and accelerating the molecular breeding of novel soybean varieties with ideal traits (e.g., high yield, high quality, and durable disease resistance) are included.
大豆[（林奈）梅里尔]是植物性蛋白质的来源，也是重要的油料作物和工业原料。社会变革导致对大豆的需求增加，与此同时，具有优良性状的大豆品种的培育也在增加。早期的基因编辑技术涉及锌指核酸酶和转录激活因子样效应物核酸酶，但第三代基因编辑技术使用成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白9（Cas9）。CRISPR/Cas9技术的迅速发展使其成为最有效、最直接、最经济且最便于用户使用的靶向基因编辑技术之一。本综述总结了CRISPR/Cas9技术在大豆分子育种中的应用。更具体地说，它概述了已被靶向的基因、发生的编辑类型、作用机制以及基因编辑效率。此外，还包括了关于加强和加速培育具有理想性状（如高产、优质和持久抗病性）的新型大豆品种分子育种的建议。
