Yano Yuki, Chiba Tomoki, Asahara Hiroshi
Department of Systems BioMedicine, Tokyo Medical and Dental University, Tokyo, Japan.
Department of Molecular and Experimental Medicine, The Scripps Research Institute, San Diego, CA, United States.
Front Genet. 2020 May 7;11:406. doi: 10.3389/fgene.2020.00406. eCollection 2020.
Since human and mouse Y chromosomes contain repeated sequences, it is difficult to determine the precise sequences and analyze the function of individual Y chromosome genes. Therefore, the causes of many diseases and abnormalities related to Y chromosome genes, such as male infertility, remain unclear. In this study, to elucidate the mouse Y chromosome, we enriched the mouse Y chromosome using a fluorescence-activated cell sorter (FACS) equipped with commonly used UV and blue 488 nm lasers and read the nucleotides using the Oxford Nanopore MinION long-read sequencer. This sequencing strategy allows us to cover the whole known region as well as the potential undetermined region of the Y chromosome. FACS-based chromosome enrichment and long-read sequencing are suitable for analysis of the Y chromosome sequences and may lead to further understanding of the physiological role of Y chromosome genes.
由于人类和小鼠的Y染色体包含重复序列，因此很难确定精确的序列并分析单个Y染色体基因的功能。因此，许多与Y染色体基因相关的疾病和异常情况的病因，如男性不育，仍不清楚。在本研究中，为了阐明小鼠Y染色体，我们使用配备常用紫外光和488 nm蓝色激光的荧光激活细胞分选仪（FACS）富集小鼠Y染色体，并使用牛津纳米孔MinION长读长测序仪读取核苷酸。这种测序策略使我们能够覆盖Y染色体的整个已知区域以及潜在的未确定区域。基于FACS的染色体富集和长读长测序适用于Y染色体序列分析，并可能有助于进一步了解Y染色体基因的生理作用。
