环保型天然合成银纳米粒子对药用诃子叶提取物对胰腺癌细胞和宫颈癌细胞的抑制作用。

Inhibitory Effect of Eco-Friendly Naturally Synthesized Silver Nanoparticles from the Leaf Extract of Medicinal Detarium microcarpum Plant on Pancreatic and Cervical Cancer Cells.

机构信息

Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Malaysia.

Microbiology and Immunology Department, School of Biomedical Sciences, Kampala International University, Western Campus, Ishaka-Bushenyi, Uganda.

出版信息

Asian Pac J Cancer Prev. 2020 May 1;21(5):1247-1252. doi: 10.31557/APJCP.2020.21.5.1247.

BACKGROUND

Recently, nanoparticle synthesis by eco-friendly methods has received tremendous attention due to the method advantages and also because of the application of the nanoparticles in cancer research. Therefore, in this study, we synthesized silver nanoparticles from Detarium microcarpum leaf phytochemicals and evaluated its inhibitory effect on pancreatic and cervical cancer cells.

MATERIALS AND METHODS

Silver nanoparticles (dAgNps) were synthesized by reacting phytochemicals of D. microcarpum leaves with silver nitrate for 12 hours. Cell viability assay was carried out to investigate the cytotoxic effect of dAgNps on HeLa and PANC-1 cells.

RESULTS

Scanning electron microscopy (SEM) and transmission electron microscopy(TEM) results revealed the average sizes of dAgNps are 81 nm and 84 nm respectively. The x-ray diffraction (XRD) pattern of dAgNps was similar to that of face centered cubic(fcc) structure of silver as reported by joint committee on powder diffraction standards (JCPDS) and fourier-transform infrared spectroscopy (FTIR) analysis showed that some phytochemicals of D. microcarpum such as polyphenols and flavonoids were likely involved in the reduction of Ag+ to form nanoparticles. Finally, cell viability assay revealed dAgNps inhibited PANC-1 and HeLa cell proliferations with IC50 values of 84 and 31.5 µg/ml respectively.

CONCLUSION

In conclusion, the synthesized nanoparticles from D. microcarpum leaves (dAgNps) have inhibitory effect on pancreatic and cervical cancer cells.

背景

最近，由于方法优势以及纳米粒子在癌症研究中的应用，通过环保方法合成纳米粒子受到了极大的关注。因此，在这项研究中，我们从 Detarium microcarpum 叶植物化学物质中合成了银纳米粒子，并评估了其对胰腺癌细胞和宫颈癌细胞的抑制作用。

材料与方法

通过用硝酸银与 D. microcarpum 叶的植物化学物质反应 12 小时来合成银纳米粒子（dAgNps）。进行细胞活力测定以研究 dAgNps 对 HeLa 和 PANC-1 细胞的细胞毒性作用。

结果

扫描电子显微镜（SEM）和透射电子显微镜（TEM）结果表明，dAgNps 的平均尺寸分别为 81nm 和 84nm。dAgNps 的 X 射线衍射（XRD）图谱与面心立方（fcc）结构的银相似，正如联合委员会粉末衍射标准（JCPDS）所报道的那样，傅里叶变换红外光谱（FTIR）分析表明，D. microcarpum 的一些植物化学物质，如多酚和类黄酮，可能参与了将 Ag+还原为纳米粒子的过程。最后，细胞活力测定显示 dAgNps 抑制了 PANC-1 和 HeLa 细胞的增殖，其 IC50 值分别为 84 和 31.5µg/ml。