伊朗西南部宫颈病变中存在 HSV1/2 或 EBV 感染与高危型 HPV DNA 的共存。
Co-existing of HSV1/2 or EBV Infection with the Presence of High-Risk HPV DNA in Cervical Lesions in the Southwest of Iran.
机构信息
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Asian Pac J Cancer Prev. 2020 May 1;21(5):1459-1464. doi: 10.31557/APJCP.2020.21.5.1459.
OBJECTIVE
While the vast majority of the cervical lesions have been attributed to the HPVs, the role of EBV and HSV1/2 as co-factors in the progression of these abnormalities needs more investigation. In this study, we aimed to determine the co-existence of EBV or HSV in cervical lesions infected with high-risk HPVs.
METHODS
Totally, 102 formaline-fixed cervical lesions with different pathological grades (LSIL, HSIL, and SCC) were enrolled in this study. DNA was extracted, and its integrity was examined by PCR assay. Two conventional PCRs were performed for the detection of EBV and HSV1/2 genomes in the tissue specimens. Besides, an in-house Real-Time PCR, as well as a nested PCR assays following sequencing, was performed to detect HPV genotypes in EBV or HSV positive samples. Results: The mean age of the participants was 42.8±13 years. Out of 102 samples, 32% (n=33) were confirmed to be LSIL, 42.2% (n=43) were HSIL, 22.5% (n=23) were SCC and 2.9% (n=3) were adenocarcinoma. EBV genome was detected in 13(12.7%) samples including 2 of LSIL, 8 of HSIL and 3 of SCC. All EBV positive samples harbored high risk HPV types 16,18 and/or 31 co-infections. However, the HSV genome was not found in any of the samples.
CONCLUSION
Our result revealed that the frequency of EBV infection is higher in HISL than LSIL. Moreover, the amount of HPV load showed an elevated level among co-infected patients, which indicates that EBV might be an enhancing factor of disease progression. In contrast, HSV may not has a role as a co-factor in cervical lesions pathogenesis.
目的
虽然绝大多数宫颈病变都归因于 HPV，但 EBV 和 HSV1/2 作为这些异常进展的协同因子的作用需要更多的研究。在这项研究中，我们旨在确定感染高危型 HPV 的宫颈病变中 EBV 或 HSV 的共存情况。
方法
本研究共纳入 102 例具有不同病理分级（LSIL、HSIL 和 SCC）的福尔马林固定宫颈病变。提取 DNA，并用 PCR 检测其完整性。对组织标本进行了两种常规 PCR 检测 EBV 和 HSV1/2 基因组。此外，还进行了内部实时 PCR 和 EBV 或 HSV 阳性样本的巢式 PCR 测序检测 HPV 基因型。
结果
参与者的平均年龄为 42.8±13 岁。在 102 例样本中，32%（n=33）被确认为 LSIL，42.2%（n=43）为 HSIL，22.5%（n=23）为 SCC，2.9%（n=3）为腺癌。13 例（12.7%）样本中检测到 EBV 基因组，包括 2 例 LSIL、8 例 HSIL 和 3 例 SCC。所有 EBV 阳性样本均携带高危 HPV 型 16、18 和/或 31 共感染。然而，在任何样本中均未发现 HSV 基因组。
结论
我们的结果表明，HSIL 中 EBV 感染的频率高于 LSIL。此外，在合并感染患者中 HPV 负荷量升高，表明 EBV 可能是疾病进展的促进因素。相比之下，HSV 可能不是宫颈病变发病机制中的协同因子。
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