College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Ecotoxicol Environ Saf. 2020 Sep 1;200:110769. doi: 10.1016/j.ecoenv.2020.110769. Epub 2020 May 24.
In recent years, with the elimination of brominated flame retardants (BFRs), the product volume of tris(2-chloroethyl) phosphate (TCEP), as a main substitute of BFRs, was increasing and frequently detected in natural waters. However, the current toxicological studies on TCEP were mainly focused on the partial life stage assessment of model animals, and thus it might underestimate the impact of TCEP on environmental risks. Therefore, the whole-life-stage effects of TCEP on growth, reproduction, survival and gene transcription in Daphnia magna (D. magna) were studied in this study after exposure to environmentally relevant or greater concentrations (500 or 5000 ng/L). It was found that chronic exposure to TCEP at environmental relevant or greater concentrations promoted growth of D. magna and the expressions of genes involved in the pathways associated with growth were significantly up-regulated. TCEP did not affect reproduction of D. magna, but the expressions of some genes screened in reproduction stage were significantly changed. Furthermore, the expressions of genes involved in two heart disease-related pathways were down-regulated at the death stage of D. magna after TCEP exposure for 62 days, suggesting that TCEP delayed the death of D. magna by retarding their heart senility.
近年来，随着溴系阻燃剂（BFRs）的淘汰，磷酸三（2-氯乙基）酯（TCEP）作为 BFRs 的主要替代品，其产品体积不断增加，并频繁在天然水中被检测到。然而，目前关于 TCEP 的毒理学研究主要集中在模式动物的部分生命阶段评估上，因此可能低估了 TCEP 对环境风险的影响。因此，本研究在环境相关浓度或更高浓度（500 或 5000ng/L）下，研究了 TCEP 对大型溞（Daphnia magna）生长、繁殖、存活和基因转录的全生命周期效应。结果发现，慢性暴露于环境相关浓度或更高浓度的 TCEP 促进了大型溞的生长，与生长相关途径的基因表达显著上调。TCEP 对大型溞的繁殖没有影响，但在繁殖阶段筛选出的一些基因的表达显著改变。此外，在 TCEP 暴露 62 天后，大型溞死亡阶段与两种心脏病相关途径相关的基因表达下调，表明 TCEP 通过延缓心脏衰老来延迟大型溞的死亡。
