College of Fisheries, Huazhong Agricultural University , Wuhan 430070, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210089, China.
Environ Sci Technol. 2015 Nov 3;49(21):12975-83. doi: 10.1021/acs.est.5b03294. Epub 2015 Oct 13.
The synthetic flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in natural waters, and its maximum concentration ever reported is 377 ng/L. However, information on the adverse effects of environmentally relevant concentrations of TDCIPP on aquatic organisms are totally unknown. In this study, <12-h old water fleas, D. magna, were exposed to concentrations of 0, 65±7.1, 550±33, or 6500±1400 ng/L TDCIPP, and dose- and time-dependent effects on reproduction and development were evaluated. Sequences of genes of D. magna were obtained from the National Center for Biotechnology Information and were used to develop PCR arrays for D. magna. Arrays were then used to study transcriptional responses of D. magna to TDCIPP. Exposure to environmentally relevant concentrations of TDCIPP significantly decreased fecundity as well as length of F0 and F1 generations. Transcriptional responses showed that, of the 155 genes tested, expressions of 57 genes were significantly changed, and some changes occurred following exposure to environmentally relevant concentrations (i.e., 65±7.1 and 550±23 ng/L). Furthermore, pathways related to protein synthesis and metabolism and endocytosis were considered to be significantly affected in a dose- and time-dependent manner and might be responsible for TDCIPP-induced reproductive and developmental toxicities.
合成阻燃剂三（1,3-二氯-2-丙基）磷酸酯（TDCIPP）在天然水中经常被检测到，其报告的最高浓度为 377ng/L。然而，关于环境相关浓度的 TDCIPP 对水生生物的不良影响的信息完全未知。在这项研究中，<12 小时大的水蚤，D. magna，暴露于 0、65±7.1、550±33 或 6500±1400ng/L TDCIPP 的浓度下，评估了繁殖和发育的剂量和时间依赖性影响。D. magna 的基因序列从国家生物技术信息中心获得，并用于开发 D. magna 的 PCR 阵列。然后，该阵列用于研究 D. magna 对 TDCIPP 的转录反应。暴露于环境相关浓度的 TDCIPP 显著降低了繁殖力以及 F0 和 F1 代的长度。转录反应表明，在测试的 155 个基因中，57 个基因的表达显著改变，并且一些变化发生在暴露于环境相关浓度（即 65±7.1 和 550±23ng/L）之后。此外，与蛋白质合成和代谢以及内吞作用相关的途径被认为以剂量和时间依赖的方式受到显著影响，并且可能是 TDCIPP 诱导的生殖和发育毒性的原因。
