Peng Guangquan, Qin Yanghe, Yan Zhiming, He Mingwei, Zhao Yucheng, Jiang Neng, Wei Changhong
Department of Research & Clinical Laboratory, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning, China.
Department of Pharmacy, Guangxi Medical University Cancer Hospital, Nanning, 530021, Guangxi, PR China.
Sci Data. 2025 Jun 2;12(1):931. doi: 10.1038/s41597-025-05058-w.
Earthworms have been extensively studied as ancient soil invertebrates, that are highly diverse. Previous studies of these invertebrates have mainly focused on their ecosystem services, medicinal value, and ecological habits. However, their genomic analysis remains inadequate. In this study, we generated the first high-quality telomere-to-telomere (T2T) assembly of the genome of the earthworm, Amynthas aspergillum (Perrier, 1872), which belongs to the genus Amynthas of the family Megascolecidae. The T2T assembly was 758.86 Mb with an N50 contig size of 16.59 Mb. The sequences were anchored to 43 chromosomes (2n = 2x = 86) with a coverage of 98.43% (746.95 Mb), and 83 telomeres were detected. In addition, we also predicted 35,723 protein-coding genes with 97.02% being functionally annotated. This T2T genome assembly will establish substantial groundwork for exploring the evolutionary mechanisms of the earthworm genome and enhance the specificity of its pharmacological effects.
蚯蚓作为古老的土壤无脊椎动物，种类极为丰富，已得到广泛研究。此前对这些无脊椎动物的研究主要集中在它们的生态系统服务、药用价值和生态习性上。然而，它们的基因组分析仍显不足。在本研究中，我们首次对蚯蚓参状远盲蚓（Perrier，1872年）的基因组进行了高质量的端粒到端粒（T2T）组装，参状远盲蚓属于巨蚓科远盲蚓属。该T2T组装基因组大小为758.86 Mb，重叠群N50大小为16.59 Mb。这些序列被定位到43条染色体（2n = 2x = 86）上，覆盖度为98.43%（746.95 Mb），并检测到83个端粒。此外，我们还预测了35723个蛋白质编码基因，其中97.02%具有功能注释。这种T2T基因组组装将为探索蚯蚓基因组的进化机制奠定坚实基础，并提高其药理作用的特异性。
