Abreu-Pereira César Augusto, Gorayb-Pereira Ana Luiza, Menezes Noveletto João Vinícius, Jordão Cláudia Carolina, Pavarina Ana Cláudia
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-385, Brazil.
J Fungi (Basel). 2023 May 16;9(5):576. doi: 10.3390/jof9050576.
This study assessed the effect of zerumbone (ZER) against fluconazole-resistant (CaR) and -susceptible (CaS) biofilms and verified the influence of ZER on extracellular matrix components. Initially, to determine the treatment conditions, the minimum inhibitory concentration (MIC), the minimum fungicidal concentration (MFC) and the survival curve were evaluated. Biofilms were formed for 48 h and exposed to ZER at concentrations of 128 and 256 µg/mL for 5, 10 and 20 min ( = 12). One group of biofilms did not receive the treatment in order to monitor the effects. The biofilms were evaluated to determine the microbial population (CFU/mL), and the extracellular matrix components (water-soluble polysaccharides (WSP), alkali-soluble polysaccharides (ASPs), proteins and extracellular DNA (eDNA), as well as the biomass (total and insoluble) were quantified. The MIC value of ZER for CaS was 256 μg/mL, and for CaR, it was 64 μg/mL. The survival curve and the MFC value coincided for CaS (256 μg/mL) and CaR (128 μg/mL). ZER reduced the cellular viability by 38.51% for CaS and by 36.99% for CaR. ZER at 256 µg/mL also reduced the total biomass (57%), insoluble biomass (45%), WSP (65%), proteins (18%) and eDNA (78%) of CaS biofilms. In addition, a reduction in insoluble biomass (13%), proteins (18%), WSP (65%), ASP (10%) and eDNA (23%) was also observed in the CaR biofilms. ZER was effective against fluconazole-resistant and -susceptible biofilms and disturbed the extracellular matrix.
本研究评估了莪术二酮（ZER）对氟康唑耐药（CaR）和敏感（CaS）生物膜的作用，并验证了ZER对细胞外基质成分的影响。首先，为确定治疗条件，评估了最低抑菌浓度（MIC）、最低杀菌浓度（MFC）和存活曲线。生物膜形成48小时后，分别用128和256μg/mL浓度的ZER处理5、10和20分钟（ = 12）。一组生物膜不接受处理以监测效果。对生物膜进行评估以确定微生物数量（CFU/mL），并对细胞外基质成分（水溶性多糖（WSP）、碱溶性多糖（ASP）、蛋白质和细胞外DNA（eDNA））以及生物量（总生物量和不溶性生物量）进行定量。ZER对CaS的MIC值为256μg/mL，对CaR为64μg/mL。CaS（256μg/mL）和CaR（128μg/mL）的存活曲线和MFC值一致。ZER使CaS的细胞活力降低38.51%，使CaR的细胞活力降低36.99%。256μg/mL的ZER还降低了CaS生物膜的总生物量（57%）、不溶性生物量（45%）、WSP（65%）、蛋白质（18%）和eDNA（78%）。此外，在CaR生物膜中也观察到不溶性生物量（13%）、蛋白质（18%）、WSP（65%））、ASP（10%）和eDNA（23%）的减少。ZER对氟康唑耐药和敏感生物膜均有效，并扰乱了细胞外基质。
