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新种和新种,从盐湖中分离得到。

sp. nov. and sp. nov., isolated from a saline lake.

机构信息

University of Chinese Academy of Sciences, Beijing, 100049, PR China.

State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Jun;70(6):3785-3793. doi: 10.1099/ijsem.0.004237. Epub 2020 May 28.

Abstract

Two novel strains, designated SYSU L10167 and SYSU L10180, were isolated from sediment sampled at Dabancheng saline lake in Xinjiang, PR China. A polyphasic approach was used to clarify the taxonomic positions of the two strains. Cells of the isolates were curved ring-like, horseshoe-shaped or rod-shaped, non-motile and non-spore-forming. Cells were Gram-stain-negative, aerobic, heterotrophic and rose-pigmented. The phylogenetic trees based on 16S rRNA gene sequences showed that strains SYSU L10167 and SYSU L10180 formed a distinct lineage within the genus . Strains SYSU L10167 and SYSU L10180 showed highest similarities to KCTC 23150 (98.0 and 97.4%, respectively). Results of genomic analyses (including average nucleotide identity, digital DNA-DNA hybridization and the marker gene tree) and pan-genome analysis further confirmed that strains SYSU L10167 and SYSU L10180 were separate from each other and other species of the genus . The draft genomes of the isolates had sizes of 5.5-5.7 Mb and reflected their major physiological capabilities. Based on phenotypic, physiological, chemotaxonomic and genotypic characterization, we propose that the isolates represent two novel species, for which the names sp. nov. and sp. nov. are proposed. The type strains of the species are SYSU L10167 (=KCTC 72390=CGMCC 1.17521) and SYSU L10180 (=KCTC 72391=CGMCC 1.17278).

摘要

两个新菌株,分别命名为 SYSU L10167 和 SYSU L10180,从中国新疆大坂城盐湖的沉积物中分离出来。采用多相分类方法来阐明这两个菌株的分类地位。分离株的细胞呈弯曲的环形、马蹄形或杆状,不运动,不形成孢子。细胞革兰氏阴性,需氧,异养,呈玫瑰色。基于 16S rRNA 基因序列构建的系统发育树显示,菌株 SYSU L10167 和 SYSU L10180 形成了一个独特的谱系,属于. 菌株 SYSU L10167 和 SYSU L10180 与 KCTC 23150 的相似度最高(分别为 98.0%和 97.4%)。基因组分析(包括平均核苷酸同一性、数字 DNA-DNA 杂交和标记基因树)和泛基因组分析的结果进一步证实,菌株 SYSU L10167 和 SYSU L10180 彼此分离,与属内的其他物种也不同。分离株的基因组草图大小为 5.5-5.7 Mb,反映了它们的主要生理能力。基于表型、生理、化学分类和基因型特征,我们建议这些分离株代表两个新的物种,分别命名为. sp. nov. 和 sp. nov. 。这两个种的模式菌株分别为 SYSU L10167(=KCTC 72390=CGMCC 1.17521)和 SYSU L10180(=KCTC 72391=CGMCC 1.17278)。

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