State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Key Laboratory of Biodiversity Conservation and Application in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, PR China.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006436.
Two Gram-stain-positive, aerobic, oxidase- and catalase-negative, non-motile, and short rod-shaped actinomycetes, named SYSU T00b441 and SYSU T00b490, were isolated from tidal flat sediment located in Guangdong province, PR China. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between SYSU T00b441 and SYSU T00b490 were 99.3, 99.5 and 97.1 %, respectively. Strains SYSU T00b441 and SYSU T00b490 exhibited the highest 16S rRNA gene sequence similarities to CF 5-4 (97.1 %/98.2 %), with ANI values of 74.01/73.88 % and dDDH values of 20.5/20.4 %. In the phylogenomic tree, the two isolates were affiliated with the genus . The genomes of strains SYSU T00b441 and SYSU T00b490 were 3.31 and 3.34 Mb, and both had DNA G+C contents of 72.8 mol%, coding 3077 and 3085 CDSs, three and three rRNA genes, and 53 and 51 tRNAs, respectively. Growth occurred at 15-40 °C (optimum, 28-30 °C), pH 4.0-10.0 (optimum, 7.0) and in the presence of 0-7 % (w/v) NaCl (optimum, 3 %). The major fatty acids (>10 %) of strains SYSU T00b441 and SYSU T00b490 were anteiso-C and C. The major respiratory quinone was identified as MK-10(H). The polar lipids of strains SYSU T00b441 and SYSU T00b490 were diphosphatidyl glycerol, phosphatidylglycerol, phosphoglycolipid, phosphatidyl ethanolamine, two phosphatidylinositol mannosides, two glycolipids and two phospholipids. Based on these data, the two strains (SYSU T00b441 and SYSU T00b490) represent a novel species of the genus , for which the name sp. nov is proposed. The type strain is SYSU T00b441 (=GDMCC 1.3827=KCTC 49943).
两株革兰氏阳性、需氧、氧化酶和过氧化氢酶阴性、不运动的短杆状放线菌，分别命名为 SYSU T00b441 和 SYSU T00b490，从中国广东省潮滩沉积物中分离得到。SYSU T00b441 和 SYSU T00b490 之间的 16S rRNA 基因序列相似度、平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 99.3%、99.5%和 97.1%。菌株 SYSU T00b441 和 SYSU T00b490 与 CF 5-4（97.1%/98.2%）的 16S rRNA 基因序列相似度最高，ANI 值分别为 74.01%/73.88%，dDDH 值分别为 20.5%/20.4%。在系统发育基因组树上，这两个分离物与属 有关。菌株 SYSU T00b441 和 SYSU T00b490 的基因组大小分别为 3.31 和 3.34 Mb，GC 含量均为 72.8 mol%，编码 3077 和 3085 个 CDS、三个和三个 rRNA 基因、53 和 51 个 tRNA。生长温度为 15-40°C（最佳温度为 28-30°C），pH 值为 4.0-10.0（最佳值为 7.0），可在 0-7%（w/v）NaCl 存在下生长（最佳值为 3%）。菌株 SYSU T00b441 和 SYSU T00b490 的主要脂肪酸（>10%）为anteiso-C 和 C。主要呼吸醌为 MK-10(H)。菌株 SYSU T00b441 和 SYSU T00b490 的极性脂为双磷脂酰甘油、磷脂酰甘油、磷酸糖脂、磷脂酰乙醇胺、两个磷壁酸甘露糖脂、两个糖脂和两个磷脂。基于这些数据，这两株（SYSU T00b441 和 SYSU T00b490）代表了一个新的属 种，提议命名为 sp. nov。模式菌株为 SYSU T00b441（=GDMCC 1.3827=KCTC 49943）。
