Sennerstam R
Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden.
J Cell Sci. 1988 Jun;90 ( Pt 2):301-6. doi: 10.1242/jcs.90.2.301.
Since the 1960s it has been thought that there is to some extent a difference in the partition of mass to daughter cells at mitosis. Recent studies using modern techniques give further support to such a phenomenon, which has become almost an axiom in cell biology. It has been suggested that such unequal distribution of metabolic constituents at mitosis contributes to the dispersion in cell generation times. In the present work, PCC3 embryonal carcinoma (EC) cells were studied as undifferentiated G1 sister pairs by microspectrophotometry (MSP) following Feulgen-Naphthol Yellow staining (FNYS), in order to evaluate their protein content. Despite the considerable intraclonal intermitotic time heterogeneity found in undifferentiated PCC3 EC cells, it was concluded thta the postmitotic difference in mass (protein) between sister cell pairs exerts a minimal influence upon the cell population mass variability, whereas it was deduced to have an influence upon variation in interphase time duration when comparing sister cell pairs. This offers a cell-physiological explanation to the randomly distributed difference repeatedly found between sister cell generation times. Furthermore, there was no correlation seen between the mass difference found between sister cell pairs postmitotically and the size of the mother cell.
自20世纪60年代以来，人们就认为在有丝分裂时母细胞质量分配到子细胞的过程在某种程度上存在差异。近期运用现代技术开展的研究进一步证实了这一现象，它几乎已成为细胞生物学中的一条公理。有人提出，有丝分裂时代谢成分的这种不均等分配会导致细胞世代时间的离散。在本研究中，通过Feulgen-萘酚黄染色（FNYS）后利用显微分光光度法（MSP），将PCC3胚胎癌细胞作为未分化的G1期姐妹细胞对进行研究，以评估其蛋白质含量。尽管在未分化的PCC3胚胎癌细胞中发现了相当大的克隆内有丝分裂间期时间异质性，但得出的结论是，姐妹细胞对之间有丝分裂后的质量（蛋白质）差异对细胞群体质量变异性的影响极小，而在比较姐妹细胞对时，推测其对间期持续时间的变化有影响。这为姐妹细胞世代时间之间反复出现的随机分布差异提供了一种细胞生理学解释。此外，有丝分裂后姐妹细胞对之间发现的质量差异与母细胞大小之间没有相关性。
