Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals andVeterinary Public Heath, University of Veterinary Medicine Vienna, Vienna, 1210, Austria.
Institute of Food Research and Product Development, University of Kasetsart, Bangkok, 10900, Thailand.
Nutrients. 2020 May 25;12(5):1542. doi: 10.3390/nu12051542.
High intestinal availability of dietary phosphorus (P) may impair calcium (Ca)homeostasis and bone integrity. In the present study, we investigated the effect of phytasesupplementation in comparison to the soaking of cereal grains in 2.5% lactic acid (LA) on intestinalCa and P absorption; intestinal, renal, and bone gene expression regarding Ca and P homeostasis;bone parameters; and serum levels of regulatory hormones in growing pigs. Thirty-two pigs wererandomly assigned to one of four diets in a 2 × 2 factorial design in four replicate batches for 19days. The diets comprised either untreated or LA-treated wheat and maize without and withphytase supplementation (500 phytase units/kg). Although both treatments improved the Pbalance, phytase and LA-treated cereals differently modulated gene expression related to intestinalabsorption, and renal and bone metabolism of Ca and P, thereby altering homeostatic regulatorymechanisms as indicated by serum Ca, P, vitamin D, and fibroblast growth factor 23 levels.Moreover, phytase increased the gene expression related to reabsorption of Ca in the kidney,whereas LA-treated cereals decreased the expression of genes for osteoclastogenesis in bones,indicating an unbalanced systemic availability of minerals. In conclusion, high intestinalavailability of dietary P may impair Ca homeostasis and bone integrity.
膳食磷（P）的高肠道可利用性可能会破坏钙（Ca）稳态和骨骼完整性。在本研究中，我们研究了植酸酶添加与谷物在 2.5%乳酸（LA）中浸泡的效果，比较了它们对肠道 Ca 和 P 吸收、涉及 Ca 和 P 稳态的肠道、肾脏和骨骼基因表达、骨骼参数以及生长猪的调节激素血清水平的影响。32 头猪被随机分配到四个处理组中的一个，采用 2×2 析因设计，在四个重复批次中进行了 19 天的试验。日粮包括未经处理或用 LA 处理的小麦和玉米，以及添加或不添加植酸酶（500 植酸酶单位/千克）。虽然两种处理都提高了磷平衡，但植酸酶和 LA 处理的谷物对与肠道吸收以及肾脏和骨骼 Ca 和 P 代谢相关的基因表达有不同的调节作用，从而改变了血清 Ca、P、维生素 D 和成纤维细胞生长因子 23 水平所指示的稳态调节机制。此外，植酸酶增加了与肾脏中 Ca 重吸收相关的基因表达，而 LA 处理的谷物降低了骨骼中破骨细胞生成的基因表达，表明矿物质的系统可用性不平衡。总之，膳食 P 的高肠道可利用性可能会破坏 Ca 稳态和骨骼完整性。
