Department of Pharmacognosy, University of Medicine and Pharmacy "Victor Babeş", Eftimie Murgu Square, No. 2, 300041 Timişoara, Romania.
Department of Pharmacology and Clinical Pharmacy, University of Medicine and Pharmacy "Victor Babeş", Eftimie Murgu Square, No. 2, 300041 Timişoara, Romania.
Biomolecules. 2020 May 26;10(6):818. doi: 10.3390/biom10060818.
L. (OB), also commonly known as evening primrose, belongs to the Onagraceae family and has the best studied biological activity of all the members in the family. In therapy, the most frequently used type of extracts are from the aerial part, which are the fatty oils obtained from the seeds and have a wide range of medicinal properties. The aim of this study was to evaluate the phytochemical composition and biological activity of OB hydroalcoholic extract and to provide directions for the antimicrobial effect, antiproliferative and pro-apoptotic potential against A375 melanoma cell line, and anti-angiogenic and anti-inflammatory capacity. The main polyphenols and flavonoids identified were gallic acid, caffeic acid, epicatechin, coumaric acid, ferulic acid, rutin and rosmarinic acid. The total phenolic content was 631.496 µgGAE/mL of extract and the antioxidant activity was 7258.67 μmolTrolox/g of extract. The tested extract had a mild bacteriostatic effect on the tested bacterial strains. It was bactericidal only against Candida spp. and S. aureus. In the set of experimental conditions, the OB extract only manifested significant antiproliferative and pro-apoptotic activity against the A375 human melanoma cell line at the highest tested concentration, namely 60 μg/mL. The migration potential of A375 cells was hampered by the OB extract in a concentration-dependent manner. Furthermore, at the highest tested concentration, the OB extract altered the mitochondrial function in vitro, while reducing the angiogenic reaction, hindering compact tumor formation in the chorioallantoic membrane assay. Moreover, the OB extract elicited an anti-inflammatory effect on the experimental animal model of ear inflammation.
月见草（L.OB），俗称晚樱草，属于柳叶菜科，其生物活性是该科所有成员中研究得最好的。在治疗中，最常使用的提取物类型来自地上部分，即从种子中获得的脂肪油，具有广泛的药用特性。本研究旨在评估 OB 水醇提取物的植物化学成分和生物活性，并为其抗菌作用、抗增殖和促凋亡作用对 A375 黑色素瘤细胞系的潜在作用，以及抗血管生成和抗炎能力提供方向。鉴定出的主要多酚和类黄酮为没食子酸、咖啡酸、表儿茶素、香豆酸、阿魏酸、芦丁和迷迭香酸。提取物中总酚含量为 631.496 µgGAE/mL，抗氧化活性为 7258.67 μmolTrolox/g 提取物。测试的提取物对测试的细菌菌株具有温和的抑菌作用。它仅对念珠菌属和金黄色葡萄球菌具有杀菌作用。在实验条件下，OB 提取物仅在最高测试浓度（60μg/mL）下对人黑色素瘤 A375 细胞系表现出显著的抗增殖和促凋亡活性。OB 提取物以浓度依赖的方式阻碍 A375 细胞的迁移潜力。此外，在最高测试浓度下，OB 提取物改变了体外的线粒体功能，同时减少了血管生成反应，阻碍了在鸡胚尿囊膜试验中肿瘤的致密形成。此外，OB 提取物对耳炎实验动物模型表现出抗炎作用。
