Raveschot Cyril, Coutte François, Frémont Marc, Vaeremans Maxime, Dugersuren Jamyan, Demberel Shirchin, Drider Djamel, Dhulster Pascal, Flahaut Christophe, Cudennec Benoit
Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France; VF Bioscience, Parc Eurasanté, F-59120 Loos-lez-Lille, France.
Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
Food Res Int. 2020 Jul;133:109201. doi: 10.1016/j.foodres.2020.109201. Epub 2020 Mar 30.
The aim of this study was to investigate the probiotic properties of 174 Lactobacillus strains isolated from Mongolian dairy products, and particularly their impact on intestinal calcium uptake and absorption. All isolates underwent a first screening based on cell surface hydrophobicity, acid tolerance, tolerance to gastro-intestinal digestion, autoaggregation, adhesion and cytotoxicity against intestinal cells. Six Lactobacillus strains from different species (L. casei, L. kefiranofaciens, L. plantarum, L. fermentum, L. helveticus and L. delbrueckii) were selected, and their impact on intestinal calcium uptake and transport was investigated using Caco-2. Five strains were able to improve total calcium transport after 24 h contact with a differentiated Caco-2 cell monolayer. Concomitantly the L. plantarum strain was able to increase cellular calcium uptake in Caco-2 cells by 10% in comparison to control conditions. To determine which pathway(s) of calcium absorption was modulated by the strains, a qPCR-based study on 4 genes related to calcium/vitamin D metabolism or tight junction integrity was conducted on mucus-secreting intestinal HT-29 MTX cells. The L. plantarum strain modulates the transcellular pathway by regulating the expression of vitamin D receptor (1.79 fold of control) and calcium transporter (4.77 fold of control) while the L. delbrueckii strain acts on the paracellular pathway by modulating claudin-2 expression (2.83 fold of control). This work highlights the impact of Lactobacillus probiotic strains on intestinal calcium absorption and for the first time give some evidence about the cellular pathways involved.
本研究的目的是调查从蒙古乳制品中分离出的174株乳酸杆菌的益生菌特性，特别是它们对肠道钙摄取和吸收的影响。所有分离株都基于细胞表面疏水性、耐酸性、对胃肠消化的耐受性、自聚集、粘附以及对肠道细胞的细胞毒性进行了初步筛选。从不同物种中挑选出6株乳酸杆菌（干酪乳杆菌、开菲尔乳杆菌、植物乳杆菌、发酵乳杆菌、瑞士乳杆菌和德氏乳杆菌），并使用Caco-2细胞研究它们对肠道钙摄取和转运的影响。5株菌株在与分化的Caco-2细胞单层接触24小时后能够改善总钙转运。同时，与对照条件相比，植物乳杆菌菌株能够使Caco-2细胞中的细胞钙摄取增加10%。为了确定菌株调节了钙吸收的哪些途径，对分泌黏液的肠道HT-29 MTX细胞中与钙/维生素D代谢或紧密连接完整性相关的4个基因进行了基于qPCR的研究。植物乳杆菌菌株通过调节维生素D受体（对照的1.79倍）和钙转运蛋白（对照的4.77倍）的表达来调节跨细胞途径，而德氏乳杆菌菌株通过调节claudin-2表达（对照的2.83倍）作用于细胞旁途径。这项工作突出了乳酸杆菌益生菌菌株对肠道钙吸收的影响，并首次提供了有关所涉及的细胞途径的一些证据。
