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通过HPLC-ESI-Q-TOF-MS/MS对2型糖尿病大鼠中增液汤的吸收和代谢成分进行系统分析与鉴定。

Systematic analysis and identification of the absorption and metabolic components of Zengye decoction in type 2 diabetic rats by HPLC-ESI-Q-TOF-MS/MS.

作者信息

Chang Shanquan, Wang Mei, Tian Yushan, Qi Jin, Qiu Zhixia

机构信息

1Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #639 Longmian Avenue, Jiangning District, Nanjing, 211198 People's Republic of China.

2Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #639 Longmian Avenue, Jiangning District, Nanjing, 211198 People's Republic of China.

出版信息

Chin Med. 2020 May 20;15:50. doi: 10.1186/s13020-020-00331-z. eCollection 2020.

DOI:10.1186/s13020-020-00331-z
PMID:32467722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7238542/
Abstract

BACKGROUND

Zengye decoction (ZYD) has been widely used in the treatment of type 2 diabetes mellitus (T2DM). Exploring the fate of various components of ZYD in vivo is of considerable significance for pharmacological research and molecular mechanism elaboration. However, the systematic analysis on the metabolic behavior of chemical components of ZYD in T2DM rats has not been reported.

METHODS

To screen and characterize the complex chemical compositions of ZYD, and metabolism fate in plasma, urine, bile, and feces of T2DM rats, the model of T2DM rats was prepared. A rapid procedure using high-performance liquid chromatography coupled with electrospray ionization quadrupole time of flight tandem mass spectrometry (HPLC-ESI-Q-TOF-MS/MS) was established. Data were acquired and analyzed by Agilent MassHunter Workstation Qualitative Analysis software version B.07.00 and PCDL manager B.07.00.

RESULTS

A total of 80 compounds were identified or tentatively characterized in ZYD, 31 more than previously detected. Besides, 36 prototype components and 49 metabolites of ZYD were found and characterized in T2DM rats, and the proposed fragmentation pathways and possible metabolic behaviors of the main types of compounds were described.

CONCLUSIONS

This study developed the understanding of the composition of ZYD as well as the cleavage rules and metabolic pathways of the prototype compounds. Besides, this study provided abundant data for further research and for study of the metabolism of traditional Chinese medicine prescriptions.

摘要

背景

增液汤（ZYD）已被广泛用于治疗2型糖尿病（T2DM）。探究增液汤各成分在体内的去向对于药理研究和阐明分子机制具有重要意义。然而，尚未见关于增液汤化学成分在T2DM大鼠体内代谢行为的系统分析报道。

方法

为筛选和表征增液汤的复杂化学成分及其在T2DM大鼠血浆、尿液、胆汁和粪便中的代谢去向，制备了T2DM大鼠模型。建立了一种使用高效液相色谱-电喷雾电离四极杆飞行时间串联质谱（HPLC-ESI-Q-TOF-MS/MS）的快速分析方法。数据通过安捷伦MassHunter工作站定性分析软件版本B.07.00和PCDL管理器B.07.00进行采集和分析。

结果

在增液汤中总共鉴定或初步表征了80种化合物，比之前检测到的多31种。此外，在T2DM大鼠中发现并表征了36种增液汤原型成分和49种代谢产物，并描述了主要类型化合物的推测裂解途径和可能的代谢行为。

结论

本研究增进了对增液汤成分以及原型化合物裂解规则和代谢途径的理解。此外，本研究为进一步研究及中药复方代谢研究提供了丰富的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/a351941a83df/13020_2020_331_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/439243539e0f/13020_2020_331_Fig1a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/43941de2bad2/13020_2020_331_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/ffef17b9bcd2/13020_2020_331_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/3087a8298d30/13020_2020_331_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/58a2062d2405/13020_2020_331_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/a351941a83df/13020_2020_331_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/439243539e0f/13020_2020_331_Fig1a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/43941de2bad2/13020_2020_331_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/ffef17b9bcd2/13020_2020_331_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/3087a8298d30/13020_2020_331_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/58a2062d2405/13020_2020_331_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b3a/7238542/a351941a83df/13020_2020_331_Fig6_HTML.jpg

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