Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Bull Environ Contam Toxicol. 2020 Jul;105(1):119-126. doi: 10.1007/s00128-020-02892-z. Epub 2020 May 28.
Cerium oxide nanoparticles (CeO NPs) are widely used in industries and have caused environmental problems. However, the phytotoxicity induced by CeO NPs lacks detailed information on phytotoxicity. In this research, the effect of CeO NPs on soybean plants (Glycine max) was studied. Scanning electron microscopy with the energy dispersion spectroscopy was used to characterize the NPs form in soybean. The growth of the root was increased, whereas the growth of shoot was inhibited. Besides, Chlorophyll Fluorescence Imager (CF Imager) showed that chlorophyll synthesis was inhibited: the maximum quantum yield of Photosystem II complex (PSII) (Fv/Fm) and photochemical quenching (qP) decreased. Moreover, transmission electron microscopy revealed that the chloroplast thylakoid structure was changed, and thus reduced the energy conversion in the Calvin cycle from C5 to C3. Our work suggests that CeO NPs will cause growth changes as well as irreversible damage to soybean plants. Our findings will provide evidence for estimation of plant toxicity induced by CeO NPs.
氧化铈纳米颗粒（CeO NPs）被广泛应用于工业生产，由此带来的环境问题也备受关注。然而，CeO NPs 的植物毒性的相关信息还不完整。本研究旨在探究 CeO NPs 对大豆植株的影响。利用扫描电子显微镜和能谱仪对大豆中的 NPs 形态进行了表征。结果表明，CeO NPs 处理后大豆的根长增加，而茎长受到抑制。此外，叶绿素荧光成像仪（CF Imager）显示 CeO NPs 抑制了叶绿素的合成：最大光化学量子产量（Fv/Fm）和光化学猝灭（qP）降低。透射电子显微镜显示，CeO NPs 处理后叶绿体的类囊体结构发生改变，从而降低了卡尔文循环中从 C5 到 C3 的能量转化。本研究表明 CeO NPs 会引起大豆植株的生长变化，并对其造成不可逆的损伤。本研究结果为评估 CeO NPs 引起的植物毒性提供了依据。
