Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
PLoS One. 2013 Apr 23;8(4):e61905. doi: 10.1371/journal.pone.0061905. Print 2013.
To better understand the role of protein synthesis in axons, we have identified the source of a portion of axonal RNA. We show that proximal segments of transected sciatic nerves accumulate newly-synthesized RNA in axons. This RNA is synthesized in Schwann cells because the RNA was labeled in the complete absence of neuronal cell bodies both in vitro and in vivo. We also demonstrate that the transfer is prevented by disruption of actin and that it fails to occur in the absence of myosin-Va. Our results demonstrate cell-to-cell transfer of RNA and identify part of the mechanism required for transfer. The induction of cell-to-cell RNA transfer by injury suggests that interventions following injury or degeneration, particularly gene therapy, may be accomplished by applying them to nearby glial cells (or implanted stem cells) at the site of injury to promote regeneration.
为了更好地理解蛋白质合成在轴突中的作用，我们已经确定了一部分轴突 RNA 的来源。我们表明，切断的坐骨神经的近端段在轴突中积累新合成的 RNA。这种 RNA 是在施万细胞中合成的，因为在体外和体内，完全不存在神经元胞体的情况下，这种 RNA 被标记。我们还证明，肌动蛋白的破坏阻止了这种转移，并且在缺乏肌球蛋白-Va 的情况下，这种转移不会发生。我们的结果表明 RNA 的细胞间转移，并确定了转移所需的部分机制。损伤诱导的细胞间 RNA 转移表明，损伤后或退化后的干预措施，特别是基因治疗，可以通过将它们应用于损伤部位的附近胶质细胞（或植入的干细胞）来完成，以促进再生。
