Ceballos-Garzon Andrés, Amado Daniela, Vélez Norida, Jiménez-A María José, Rodríguez Crescencio, Parra-Giraldo Claudia Marcela
Unidad de Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C. 110231, Colombia.
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá 110931, Colombia.
J Fungi (Basel). 2020 May 27;6(2):72. doi: 10.3390/jof6020072.
Background: is characterized for having a high genetic variability among species. MALDI-TOF MS library contains spectra from only three strains of , which makes difficult the identification process and gives low scores at the species level. Our aim was to construct and validate an internal library to improve identification with Colombian clinical strains.
From 30 clinical strains, 770 mass spectra were obtained for the construction of the database. The validation was performed with 300 strains to compare the identification results in the BDAL and Colombia libraries.
Our library allowed a complete, 100% identification of the evaluated strains and a significant improvement in the scores obtained, showing a better performance compared to the Bruker BDAL library.
The strengthening of the database is a great opportunity to improve the scoring and identification. Library data are available via ProteomeXchange with identifier PXD016387.
背景：其特点是物种间具有高度遗传变异性。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）文库仅包含来自三种菌株的光谱，这使得鉴定过程困难，且在物种水平上得分较低。我们的目的是构建并验证一个内部文库，以改进对哥伦比亚临床菌株的鉴定。
从30株临床菌株中获得770个质谱用于构建数据库。用300株菌株进行验证，以比较在BDAL和哥伦比亚文库中的鉴定结果。
我们的文库能够对评估菌株进行100%的完整鉴定，且获得的分数有显著提高，与布鲁克BDAL文库相比表现更好。
加强数据库是提高评分和鉴定的好机会。文库数据可通过ProteomeXchange获得，标识符为PXD016387。
