Department of Thoracic Surgery, Caoxian People's Hospital, Heze, China.
Department of Respiratory Medicine, Caoxian People's Hospital, Heze, China.
Exp Mol Pathol. 2020 Aug;115:104476. doi: 10.1016/j.yexmp.2020.104476. Epub 2020 May 28.
To uncover the expression pattern and the prognosis of miR-4262 in these patients with esophageal cancer, and its potential mechanism.
MiR-4262 levels in 57 esophageal cancer and paracancerous specimens were detected. The relationship between miR-4262 level and clinical features of esophageal cancer was analyzed. After overexpression of miR-4262 in OE19 and EC-109 cells, changes in proliferative potential and apoptosis were examined. The interaction between miR-4262 and KLF6 was explored by dual-luciferase reporter assay. Their involvement in the development of esophageal cancer was finally determined.
MiR-4262 was downregulated in esophageal cancer specimens and cell lines. Low level of miR-4262 predicted advanced pathological staging and poor prognosis in esophageal cancer patients. Overexpression of miR-4262 reduced proliferative potential and enhanced apoptosis in esophageal cancer cells. KLF6 was the downstream gene binding to miR-4262. The interaction between miR-4262 and KLF6 was responsible for alleviating the malignant development of esophageal cancer.
MiR-4262 is downregulated in esophageal cancer and linked to its pathological staging and prognosis. MiR-4262 inhibits the malignant development of esophageal cancer by down-regulating KLF6.
揭示 miR-4262 在这些食管癌患者中的表达模式和预后及其潜在机制。
检测 57 例食管癌及癌旁标本中 miR-4262 水平。分析 miR-4262 水平与食管癌临床特征的关系。在 OE19 和 EC-109 细胞中转染过表达 miR-4262，检测增殖能力和凋亡的变化。通过双荧光素酶报告基因实验探索 miR-4262 与 KLF6 的相互作用。最后确定它们在食管癌发展中的作用。
miR-4262 在食管癌标本和细胞系中下调。miR-4262 低表达预示食管癌患者的病理分期较晚，预后不良。过表达 miR-4262 可降低食管癌细胞的增殖能力，促进凋亡。KLF6 是与 miR-4262 结合的下游基因。miR-4262 与 KLF6 的相互作用负责减轻食管癌的恶性发展。
miR-4262 在食管癌中下调，与食管癌的病理分期和预后相关。miR-4262 通过下调 KLF6 抑制食管癌的恶性发展。
