Department of Gastroenterology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Department of General Surgery, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China.
J Clin Lab Anal. 2022 Jul;36(7):e24532. doi: 10.1002/jcla.24532. Epub 2022 Jun 13.
Circular RNAs (circRNAs) play important roles in various malignancies, such as colorectal cancer (CRC). However, the function of hsa_circ_0001550 in CRC remains to be elucidated.
The expression levels of hsa_circ_0001550, microRNA (miR)-4262, and nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1) were determined by real-time qPCR. Cell biological behaviors were evaluated via colony formation assay, transwell assay, flow cytometry, and sphere formation assays. The target relationship was validated via dual-luciferase reporter and RNA pull-down assays. Protein expression was analyzed by western blot. Xenograft tumor model was adopted to evaluate hsa_circ_0001550 function in vivo.
Hsa_circ_0001550 enrichment was enhanced in CRC tissue specimens and cell lines. Hsa_circ_0001550 absence hindered CRC cell proliferation, metastasis, stemness, and caused apoptosis. Hsa_circ_0001550 targeted miR-4262, and hsa_circ_0001550 absence-caused impacts were diminished by anti-miR-4262. MiR-4262 targeted NUCKS1. Hsa_circ_0001550 had positive regulation on NUCKS1 expression. NUCKS1 overexpression overturned the influences of hsa_circ_0001550 silencingon CRC cell progression. Hsa_circ_0001550 interference notably blocked in vivo xenograft tumor growth.
Hsa_circ_0001550 facilitated CRC progression by binding to miR-4262 to positively regulate NUCKS1 abundance.
环状 RNA（circRNA）在多种恶性肿瘤中发挥重要作用，如结直肠癌（CRC）。然而，hsa_circ_0001550 在 CRC 中的作用仍有待阐明。
通过实时 qPCR 测定 hsa_circ_0001550、微小 RNA（miR）-4262 和核钙蛋白酶和细胞周期蛋白依赖性激酶底物 1（NUCKS1）的表达水平。通过集落形成试验、Transwell 试验、流式细胞术和球体形成试验评估细胞生物学行为。通过双荧光素酶报告和 RNA 下拉测定验证靶标关系。通过 Western blot 分析蛋白表达。采用异种移植肿瘤模型在体内评估 hsa_circ_0001550 的功能。
hsa_circ_0001550 在 CRC 组织标本和细胞系中富集增加。hsa_circ_0001550 的缺失抑制了 CRC 细胞的增殖、转移、干性，并导致细胞凋亡。hsa_circ_0001550 靶向 miR-4262，抗 miR-4262 减弱了 hsa_circ_0001550 缺失引起的影响。miR-4262 靶向 NUCKS1。hsa_circ_0001550 对 NUCKS1 表达有正向调节作用。NUCKS1 过表达逆转了 hsa_circ_0001550 沉默对 CRC 细胞进展的影响。hsa_circ_0001550 干扰显著阻断体内异种移植肿瘤生长。
hsa_circ_0001550 通过与 miR-4262 结合正向调节 NUCKS1 丰度促进 CRC 进展。
