Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Biomolecular Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Curr Opin Chem Biol. 2020 Oct;58:10-19. doi: 10.1016/j.cbpa.2020.04.016. Epub 2020 May 27.
Histone post-translational modifications play significant roles in gene regulation processes. Among many approaches, chemical protein synthesis has been a successful and promising method for the preparation of homogeneous products of site-specifically modified histones for elucidation of their biological significance. In this short review, we describe the recent advances in synthetic toolbox for histone proteins such as thioester precursors, chemical ubiquitination, and one-pot peptide ligation.
组蛋白翻译后修饰在基因调控过程中发挥着重要作用。在众多方法中，化学蛋白质合成是一种成功且有前途的方法，可用于制备特异性修饰组蛋白的均一组分，以阐明其生物学意义。在这篇简短的综述中，我们描述了组蛋白蛋白质合成工具的最新进展，如硫酯前体、化学泛素化和一锅肽连接。
