Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China.
Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China; China Tobacco Sichuan Industry Co., Ltd., Chengdu 610066, PR China.
Food Chem. 2020 Oct 30;328:127121. doi: 10.1016/j.foodchem.2020.127121. Epub 2020 May 22.
Chlorine dioxide (ClO) gas was utilized for detoxifying aflatoxin B (AFB) in corn for the first time. Four degradation compounds were identified by LC-MS as CHO, CHO, CHO, and CHO. Structurally, the biological activity of ClO-treated AFB was removed due to the disappearance of C8-C9 double bond in the furan ring and the modification of cyclopentanone and methoxy after ClO treatment. The cell viability assay on human embryo hepatocytes confirmed little toxicity of the degradation products. The degradation efficiency of AFB on corn peaked near 90.0% under the optimized conditions and reached 79.6% for low initial contamination of AFB at 5-20 μg/kg. Accordingly, ClO has the potential to be developed into an effective, efficient, and economic approach to detoxify AFB in grains.
二氧化氯（ClO）气体首次被用于玉米中黄曲霉毒素 B（AFB）的解毒。通过 LC-MS 鉴定出四种降解产物为 CHO、CHO、CHO 和 CHO。结构上，由于呋喃环中 C8-C9 双键的消失以及 ClO 处理后环戊酮和甲氧基的修饰，ClO 处理的 AFB 的生物活性被去除。人胚肝细胞的细胞活力测定证实降解产物的毒性很小。在优化条件下，AFB 在玉米上的降解效率峰值接近 90.0%，对于初始污染水平为 5-20μg/kg 的低浓度 AFB，降解效率达到 79.6%。因此，ClO 有可能被开发成为一种有效、高效且经济的方法，用于解毒谷物中的 AFB。
