Lou Haiwei, Li Yang, Yang Chuangming, Li Yonghui, Gao Yiyue, Li Yujin, Zhao Renyong
College of Food Science and Engineering, Henan University of Technology, Zhengzhou, China.
Department of Grain Science and Industry, Kansas State University, Manhattan, USA.
J Sci Food Agric. 2024 Jan 30;104(2):655-663. doi: 10.1002/jsfa.12956. Epub 2023 Sep 13.
Corn, being an important grain, is prone to contamination by aflatoxin B (AFB ), and AFB -contaminated corn severely endangers the health of humans and livestock. Trametes versicolor, a fungus that can grow in corn, possesses the ability to directly degrade AFB through its laccase. This study aimed to optimize the fermentation conditions for T. versicolor to degrade AFB in corn and investigate the effect of T. versicolor fermentation on the nutritional composition of corn. AFB -contaminated corn was used as the culture substrate for T. versicolor. A combination of single-factor experiments and response surface methodology was employed to identify the optimal conditions of AFB degradation.
The optimal conditions of AFB degradation were as follows: 9 days of fermentation, a fermentation temperature of 26.7 °C, a moisture content of 70.5% and an inoculation amount of 4.9 mL (containing 51.99 mg of T. versicolor mycelia). With the optimal conditions, the degradation rate of AFB in corn could reach 93.01%, and the dry basis content of protein and dietary fiber in the fermented corn was significantly increased. More importantly, the lysine content in the fermented corn was also significantly increased.
This is the first report that direct fermentation of AFB -contaminated corn by T. versicolor not only efficiently degrades AFB but also improves the nutritional composition of corn. These findings suggest that the fermentation of corn by T. versicolor is a promising, environmentally friendly and efficient approach to degrade AFB and improve the nutritional value of corn. © 2023 Society of Chemical Industry.
玉米作为一种重要的谷物，容易受到黄曲霉毒素B（AFB）的污染，而受AFB污染的玉米会严重危害人类和牲畜的健康。云芝是一种能在玉米中生长的真菌，它具有通过漆酶直接降解AFB的能力。本研究旨在优化云芝降解玉米中AFB的发酵条件，并研究云芝发酵对玉米营养成分的影响。以受AFB污染的玉米作为云芝的培养底物，采用单因素实验和响应面法相结合的方式来确定AFB降解的最佳条件。
AFB降解的最佳条件如下：发酵9天，发酵温度26.7°C，水分含量70.5%，接种量4.9 mL（含51.99 mg云芝菌丝体）。在最佳条件下，玉米中AFB的降解率可达93.01%，发酵玉米中蛋白质和膳食纤维的干基含量显著增加。更重要的是，发酵玉米中的赖氨酸含量也显著增加。
这是首次报道云芝直接发酵受AFB污染的玉米不仅能有效降解AFB，还能改善玉米的营养成分。这些研究结果表明，云芝发酵玉米是一种有前景的、环保且高效的降解AFB和提高玉米营养价值的方法。© 2023化学工业协会。
