Bong Sun Ju, Jeon Jin, Park Yun Ji, Kim Jae Kwang, Park Sang Un
1Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134 Korea.
2Division of Life Sciences and Bio-Resource and Environmental Center, Incheon National University, Yeonsu-gu, Incheon, 22012 Korea.
3 Biotech. 2020 Jun;10(6):260. doi: 10.1007/s13205-020-02244-y. Epub 2020 May 19.
Watercress ( R. Br.) is a cruciferous plant consumed by people worldwide. This vegetable contains numerous health-benefiting compounds; however, gene information and metabolomic profiling of individual parts for this plant species are scarce. In this study, we investigated the expression patterns of phenylpropanoid biosynthetic genes and the content of phenylpropanoids in different parts of watercress. We identified 11 genes encoding enzymes related to the phenylpropanoid biosynthetic pathway and analyzed the expression patterns of these genes in the leaves, stems, roots, flowers, and seeds of watercress. The result showed that most of the genes were expressed at the highest levels in the flowers. HPLC analysis performed in samples from these same parts revealed the presence of seven phenylpropanoid-derived compounds. The content of total phenylpropanoids was the highest in flowers, followed by that in the leaves, whereas the lowest level was generally detected in the stems. Rutin was the most abundant phenylpropanoid in all plant segments, while quercetin was detected only in the flowers and roots. This study provides useful information for further molecular and functional research involving and closely related species.
豆瓣菜（R. Br.）是一种全世界人们都食用的十字花科植物。这种蔬菜含有许多有益健康的化合物；然而，关于该植物物种各个部分的基因信息和代谢组学分析却很少。在本研究中，我们调查了豆瓣菜不同部位苯丙烷类生物合成基因的表达模式以及苯丙烷类化合物的含量。我们鉴定出11个编码与苯丙烷类生物合成途径相关酶的基因，并分析了这些基因在豆瓣菜的叶、茎、根、花和种子中的表达模式。结果表明，大多数基因在花中的表达水平最高。对这些相同部位的样本进行的高效液相色谱分析显示存在7种苯丙烷类衍生化合物。总苯丙烷类化合物的含量在花中最高，其次是叶，而在茎中通常检测到的水平最低。芦丁是所有植物部位中含量最丰富的苯丙烷类化合物，而槲皮素仅在花和根中检测到。本研究为涉及该植物及其近缘物种的进一步分子和功能研究提供了有用信息。
