从培养的哺乳动物细胞中分离有丝分裂纺锤体并进行初步电泳分析。 - Suppr

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The isolation and preliminary electrophoretic analysis of the mitotic spindle from cultured mammalian cells.

作者信息

Chu L K, Sisken J E

出版信息

Exp Cell Res. 1977 Jun;107(1):71-7. doi: 10.1016/0014-4827(77)90387-1.

DOI:10.1016/0014-4827(77)90387-1

PMID:324782

Abstract

摘要

相似文献

The isolation and preliminary electrophoretic analysis of the mitotic spindle from cultured mammalian cells.从培养的哺乳动物细胞中分离有丝分裂纺锤体并进行初步电泳分析。

Exp Cell Res. 1977 Jun;107(1):71-7. doi: 10.1016/0014-4827(77)90387-1.

High molecular weight MAPs are part of the mitotic spindle.高分子量微管相关蛋白是有丝分裂纺锤体的一部分。

J Cell Biol. 1978 Apr;77(1):R9-12. doi: 10.1083/jcb.77.1.r9.

Localization of tubulin in the mitotic apparatus of mammalian cells by immunofluorescence and immunoelectron microscopy.通过免疫荧光和免疫电子显微镜对微管蛋白在哺乳动物细胞有丝分裂器中的定位

Chromosoma. 1977 Apr 19;60(3):223-35. doi: 10.1007/BF00329772.

Immunoreactive forms of human erythrocyte ankyrin are localized in mitotic structures in cultured cells and are associated with microtubules in brain.人红细胞锚蛋白的免疫反应形式定位于培养细胞的有丝分裂结构中，并与脑中的微管相关。

Cold Spring Harb Symp Quant Biol. 1982;46 Pt 2:647-57. doi: 10.1101/sqb.1982.046.01.061.

Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.微管蛋白α亚基的异质性以及单个生物体中微管蛋白的变异性。

J Cell Biol. 1976 May;69(2):301-12. doi: 10.1083/jcb.69.2.301.

Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Cytoplasmic microtubules, vinblastine-induced paracrystals, and mitotic figures.通过免疫荧光对组织培养细胞中含微管蛋白结构进行特异性可视化。细胞质微管、长春花碱诱导的副晶体和有丝分裂图像。

Exp Cell Res. 1975 Oct 1;95(1):111-20. doi: 10.1016/0014-4827(75)90615-1.

Specific visualization of the distribution of the calcium dependent regulatory protein of cyclic nucleotide phosphodiesterase (modulator protein) in tissue culture cells by immunofluorescence microscopy: mitosis and intercellular bridge.通过免疫荧光显微镜对组织培养细胞中环核苷酸磷酸二酯酶钙依赖性调节蛋白（调节蛋白）分布的特异性可视化：有丝分裂和细胞间桥。

Cytobiologie. 1978 Aug;17(2):354-64.

Immunofluorescence colocalization of the 90-kDa heat-shock protein and microtubules in interphase and mitotic mammalian cells.90-kDa热休克蛋白与间期及有丝分裂期哺乳动物细胞微管的免疫荧光共定位

Eur J Cell Biol. 1989 Oct;50(1):66-75.

Isolation of microtubule protein from cultured mouse neuroblastoma cells.从小鼠神经母细胞瘤细胞培养物中分离微管蛋白。

Proc Natl Acad Sci U S A. 1970 Jan;65(1):129-36. doi: 10.1073/pnas.65.1.129.

A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence.通过间接免疫荧光观察到的哺乳动物有丝分裂纺锤体中肌动蛋白和微管蛋白分布的比较。

J Cell Biol. 1977 Mar;72(3):552-67. doi: 10.1083/jcb.72.3.552.

引用本文的文献

The midbody and midbody remnant: from cellular debris to signaling organelle with diagnostic and therapeutic potential.中间体及中间体残余物：从细胞碎片到具有诊断和治疗潜力的信号细胞器

Mol Biol Cell. 2025 Jul 1;36(7):re4. doi: 10.1091/mbc.E25-03-0120. Epub 2025 May 28.

Isolation and initial characterization of the mammalian midbody.哺乳动物中体的分离与初步表征

J Cell Biol. 1982 Sep;94(3):654-61. doi: 10.1083/jcb.94.3.654.

Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis).从海胆胚胎（强壮球海胆）中分离出的有丝分裂纺锤体中的类动力蛋白Mg2 + -ATP酶。

J Cell Biol. 1980 Sep;86(3):738-45. doi: 10.1083/jcb.86.3.738.

Studies of a microtubule-associated protein using a monoclonal antibody elicited against mammalian mitotic spindles.一项使用针对哺乳动物有丝分裂纺锤体产生的单克隆抗体对一种微管相关蛋白进行的研究。

J Cell Biol. 1984 Aug;99(2):503-11. doi: 10.1083/jcb.99.2.503.

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