哺乳动物中体的分离与初步表征
Isolation and initial characterization of the mammalian midbody.
作者信息
Mullins J M, McIntosh J R
出版信息
J Cell Biol. 1982 Sep;94(3):654-61. doi: 10.1083/jcb.94.3.654.
Abstract
Midbodies were isolated from synchronized cultures of Chinese hamster ovary (CHO) cells and their protein composition was studied by means of SDS PAGE. Gels of the midbodies included alpha and beta tubulins as major bands (approximately 30% of the total protein) and approximately 35 other bands, none of which constituted greater than 3.5% of the total protein. Extraction of the isolated midbodies with Sarkosyl NL-30- solubilized the midbody microtubules but left the central, dense matrix zone of the midbody intact. A protein doublet of approximately 115,000 mol wt was retained preferentially by the particulate fraction containing the matrix zones, indicating it to be a component of the matrix. The 115,000 mol wt doublet was also present in gels of isolated mitotic spindles from CHO cells. The overall protein composition of the isolated spindles was very similar to that of the isolated midbodies.
摘要
从中仓鼠卵巢（CHO）细胞的同步培养物中分离出中间体，并通过SDS-PAGE研究其蛋白质组成。中间体的凝胶包含α和β微管蛋白作为主要条带（约占总蛋白的30%）以及约35条其他条带，其中没有一条条带占总蛋白的比例超过3.5%。用十二烷基肌氨酸钠NL-30提取分离出的中间体可溶解中间体微管，但中间体的中央致密基质区保持完整。大约115,000摩尔质量的蛋白质双峰被含有基质区的颗粒部分优先保留，表明它是基质的一个组成部分。115,000摩尔质量的双峰也存在于CHO细胞分离的有丝分裂纺锤体的凝胶中。分离出的纺锤体的总体蛋白质组成与分离出的中间体非常相似。
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STRUCTURE OF THE MITOTIC SPINDLE IN L STRAIN FIBROBLASTS.L株成纤维细胞有丝分裂纺锤体的结构
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