Emsen Bugrahan, Guven Busranur, Uzun Yasin, Kaya Abdullah
Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey.
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey; Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey.
Int J Med Mushrooms. 2020;22(2):161-170. doi: 10.1615/IntJMedMushrooms.2020033845.
This study reported the genetic and oxidative effects of aqueous and methanol extracts from two edible mushrooms, Lepista nuda (Bull.) Cooke and Pleurotus ostreatus (Jacq.) P. Kummer, in cultured human lymphocytes. Chromosome aberration (CA) and micronucleus (MN) assays were used for genotoxic influences estimation. In addition, the changes of total antioxidant capacity (TAC) and total oxidative stress (TOS) in the cells were monitored. The fungal extracts at all applied concentrations did not indicate significant differences (p > 0.05) in CA and MN analyses. Furthermore, while the treatments with maximum concentration of aqueous extract of L. nuda statistically (p < 0.05) increased TAC especially, TOS levels in the cells were reduced by them in comparison with negative control. Based on TAC analysis, low IC50 values belonging to aqueous (5.43 mg/L) and methanol (10.88 mg/L) extracts of L. nuda were remarkable. Our data demonstrated that the extracts obtained from P. ostreatus and especially L. nuda can be a new resource for therapeutics with their nonmutagenic and antioxidant features.
本研究报告了两种可食用蘑菇——裸口蘑（Lepista nuda (Bull.) Cooke）和平菇（Pleurotus ostreatus (Jacq.) P. Kummer）的水提取物和甲醇提取物对培养的人淋巴细胞的遗传和氧化作用。采用染色体畸变（CA）和微核（MN）试验评估遗传毒性影响。此外，监测细胞中总抗氧化能力（TAC）和总氧化应激（TOS）的变化。在所有应用浓度下，真菌提取物在CA和MN分析中均未显示出显著差异（p > 0.05）。此外，虽然裸口蘑水提取物最大浓度处理显著（p < 0.05）提高了TAC，但与阴性对照相比，细胞中的TOS水平却降低了。基于TAC分析，裸口蘑水提取物（5.43 mg/L）和甲醇提取物（10.88 mg/L）的低IC50值很显著。我们的数据表明，平菇尤其是裸口蘑的提取物具有非诱变和抗氧化特性，可成为治疗的新资源。
