Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey.
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey; Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey.
Int J Med Mushrooms. 2021;23(3):67-77. doi: 10.1615/IntJMedMushrooms.2021037653.
In this study, acetone and water extracts obtained from edible mushrooms, Rhizopogon luteolus Fr. and Rh. roseolus (Corda) Th. Fr., containing important bioactive components were used. Total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were examined on human peripheral lymphocyte culture treated with the respective extracts. Levels of genetic damage and cytotoxic effects of the respective extracts on lymphocytes were also tested. In general, when TAC levels of the extracts on cells were examined, a concentration-dependent increase was observed; a negative correlation was found between TOS data and concentration. Genotoxicity tests (chromosome aberration and micronucleus analysis) revealed that the concentrations of the extract applications did not significantly (P > 0.05) change genotoxicity on human peripheral lymphocyte culture compared to the negative control. Considering all of the results obtained, it was determined that applications of Rh. luteolus and Rh. roseolus extracts, especially at concentrations of 50 and 100 mg/L, increased the TAC value of lymphocytes, which play an important role in the human immune system, without causing genetic or oxidative stress.
在这项研究中，使用了从可食用蘑菇 Rhizopogon luteolus Fr. 和 Rh. roseolus (Corda) Th. Fr. 中提取的含有重要生物活性成分的丙酮和水提取物。研究了不同浓度的提取物对人外周血淋巴细胞培养物的总抗氧化能力 (TAC) 和总氧化应激 (TOS) 水平的影响。同时还测试了不同提取物对淋巴细胞的遗传损伤和细胞毒性作用。一般来说，当检查细胞中提取物的 TAC 水平时，观察到浓度依赖性增加；TOS 数据与浓度之间存在负相关。遗传毒性试验（染色体畸变和微核分析）表明，与阴性对照相比，提取物应用浓度对人外周血淋巴细胞培养物的遗传毒性没有显著变化（P>0.05）。考虑到所有获得的结果，确定 Rh. luteolus 和 Rh. roseolus 提取物的应用，特别是在 50 和 100mg/L 的浓度下，增加了在人体免疫系统中起重要作用的淋巴细胞的 TAC 值，而不会引起遗传或氧化应激。
