Emsen Bugrahan, Kolukisa Ayse Levent
Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey.
Z Naturforsch C J Biosci. 2020 Dec 21;76(7-8):291-299. doi: 10.1515/znc-2020-0135. Print 2021 Jul 27.
In the present study, we investigated cytogenetic and oxidative [total antioxidant capacity (TAC), total oxidant status (TOS)] effects of methanol and water extracts of (Flörke Sommerf.) Sprengel, (L.) W.Mann and (L.) Ach. on cultured human lymphocytes. In addition, different phenolic compounds in the extracts were quantified by high performance liquid chromatography (HPLC) analysis. As a result of HPLC analysis, methanol extracts of all lichen species tested had higher phenolic compounds. Likewise, methanol extracts of each lichen increased TAC levels in lymphocytes more than water extracts. The TOS levels of the cells treated with different concentrations (1-100 mg/L) of the extracts decreased due to the increasing concentration of the extracts. Genotoxicity experiments revealed that the tested lichen extracts did not significantly increase ( > 0.05) the level of genotoxicity on human peripheral lymphocyte culture compared to the negative control group. The results showed that , and lichens, which were found to be a rich source of phenolic compounds, might be of interest in the pharmaceutical and food industries.
在本研究中，我们调查了扁枝衣（Flörke & Sommerf.）Sprengel、地卷衣（L.）W.Mann和冰岛衣（L.）Ach. 的甲醇提取物和水提取物对培养的人淋巴细胞的细胞遗传学和氧化作用[总抗氧化能力（TAC）、总氧化状态（TOS）]。此外，通过高效液相色谱（HPLC）分析对提取物中的不同酚类化合物进行了定量。HPLC分析结果显示，所有测试地衣物种的甲醇提取物都含有更高的酚类化合物。同样，每种地衣的甲醇提取物比水提取物更能提高淋巴细胞中的TAC水平。随着提取物浓度的增加，用不同浓度（1 - 100 mg/L）提取物处理的细胞的TOS水平降低。遗传毒性实验表明，与阴性对照组相比，测试的地衣提取物在人外周淋巴细胞培养中并未显著增加（P > 0.05）遗传毒性水平。结果表明，被发现富含酚类化合物的扁枝衣、地卷衣和冰岛衣可能在制药和食品工业中具有应用价值。
