Maphosa Lancelot, Collins Nicholas C, Taylor Julian, Mather Diane E
Australian Centre for Plant Functional Genomics, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
Funct Plant Biol. 2014 Sep;41(9):1002-1008. doi: 10.1071/FP14060.
High temperatures during grain filling can reduce the yield of wheat and affect its grain protein concentration. The Gpc-B1 locus of wheat also affects grain protein concentration, but it is not known whether its effects interact with those of heat. The aim of this study was to investigate the effects of high temperature in lines with and without functional (high-protein) alleles at Gpc-B1. A highly replicated experiment was conducted in a glasshouse under control conditions (24/18°C, 14/10h day/night), with half of the plants of each line or cultivar put into a heat chamber (37/27°C, 14/10h day/night) at 15 days after anthesis for 3 days. Backcross derivatives with the Gpc-B1 introgression segment differed from their recurrent parents more than those without that segment. In some respects, the effects of the Gpc-B1 introgression were similar to those of the heat treatment: both could accelerate peduncle senescence, increase grain protein content and increase the percentage of unextractable polymeric protein. Unlike the heat treatment, Gpc-B1 did not reduce grain weight, indicating that factors that hasten senescence do not necessarily limit grain size. The presence of the Gpc-B1 segment did not exacerbate the effects of heat stress on any trait.
灌浆期高温会降低小麦产量并影响其籽粒蛋白质浓度。小麦的Gpc-B1基因座也会影响籽粒蛋白质浓度，但尚不清楚其效应是否与高温效应相互作用。本研究的目的是调查在有和没有Gpc-B1功能性（高蛋白）等位基因的品系中高温的影响。在温室中于对照条件（24/18°C，14/10小时昼/夜）下进行了高度重复的实验，每个品系或品种的一半植株在开花后15天放入加热室（37/27°C，14/10小时昼/夜）中处理3天。带有Gpc-B1渐渗片段的回交衍生物与其轮回亲本的差异大于没有该片段的衍生物。在某些方面，Gpc-B1渐渗的效应与热处理的效应相似：两者都可加速穗颈衰老、增加籽粒蛋白质含量并增加不可提取的聚合蛋白的百分比。与热处理不同，Gpc-B1不会降低粒重，这表明加速衰老的因素不一定会限制籽粒大小。Gpc-B1片段的存在并未加剧热胁迫对任何性状的影响。
