Chen Haiyan, Chi Xuemei, Li Bowen, Zhang Min, Ma Yuxiang, Achilefu Samuel, Gu Yueqing
Department of Biomedical Engineering, School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing 210009, China.
Biomater Sci. 2014 Jul 3;2(7):996-1006. doi: 10.1039/c3bm60323g. Epub 2014 Feb 27.
In this study, gold nanorods (AuNRs) were first stabilized by hexadecyltrimethylammonium bromide (CTAB) and then coated with two kinds of polyelectrolytes (PE) and BSA to obtain multi-layered AuNRs (AuNRs-PE-BSA). Furthermore, the anti-cancer drug doxorubicin (DOX) was encapsulated into AuNRs-PE-BSA by the electrostatic force and the nanocomposites formed were named AuNRs/DOX-PE-BSA. The success of coating was verified by transmission electron microscopy (TEM), zeta potential, gel-electrophoresis and thermogravimetric analysis (TGA). The MTT assay indicated that the cytotoxicity of AuNRs decreased dramatically after multi-layer capping. The time-dependent nucleus-targeting capability of AuNRs/DOX-PE-BSA was confirmed in cell affinity evaluations. The in vitro and in vivo experiments demonstrated that AuNRs/DOX-PE-BSA, which combined photothermal and chemotherapy for tumor therapy, bears a markedly improved curative effect and holds promising prospects in the field of nanomedicine.
在本研究中，金纳米棒（AuNRs）首先用十六烷基三甲基溴化铵（CTAB）进行稳定，然后用两种聚电解质（PE）和牛血清白蛋白（BSA）进行包覆，以获得多层金纳米棒（AuNRs-PE-BSA）。此外，通过静电力将抗癌药物阿霉素（DOX）封装到AuNRs-PE-BSA中，形成的纳米复合材料命名为AuNRs/DOX-PE-BSA。通过透射电子显微镜（TEM）、zeta电位、凝胶电泳和热重分析（TGA）验证了包覆的成功。MTT分析表明，多层包覆后AuNRs的细胞毒性显著降低。在细胞亲和力评估中证实了AuNRs/DOX-PE-BSA的时间依赖性核靶向能力。体外和体内实验表明，将光热疗法和化疗相结合用于肿瘤治疗的AuNRs/DOX-PE-BSA具有显著提高的疗效，在纳米医学领域具有广阔的前景。
