Kim Kijeong, Sung Yun-Hee, Seo Jin-Hee, Lee Sang-Won, Lim Baek-Vin, Lee Choong-Yeol, Chung Yong-Rak
School of Exercise & Sport Science, College of Natural Sciences, University of Ulsan, Ulsan, Korea.
Department of Physical Therapy, College of Natural Science, Kyungnam University, Changwon, Korea.
J Exerc Rehabil. 2015 Dec 31;11(6):296-302. doi: 10.12965/jer.150264. eCollection 2015 Dec.
Maternal infection is an important factor causing neonatal brain injury and later developmental disability. In the present study, we investigated the effects of treadmill exercise intensity on short-term memory, hippocampal neurogenesis, and expression of brain-derived neurotrophic factor (BDNF), and tyrosine kinase receptor B (TrkB) in the rats born of lipopolysaccharide (LPS)-exposed maternal rats. The rats were divided into six groups: control group, mild-intensity exercise group, moderate-intensity exercise group, maternal LPS-exposed group, maternal LPS-exposed and mild-intensity exercise group, maternal LPS-exposed and moderate-intensity exercise group. The rats in the exercise groups were forced to run on a treadmill for 30 min 5 times a week for 4 weeks. The exercise load consisted of running at the speed of 8 m/min for the mild-intensity exercise groups and 14 m/min for moderate-intensity exercise groups. The latency in the step-down avoidance task was deter-mined for the short-term memory. Immunohistochemistry for 5-bro-mo-2'-deoxyuridine was performed to determine hippocampal cell proliferation and neurogenesis. Western blot analysis was performed for the detection of BDNF and TrkB expression. In the present study, tread-mill exercise improved short-term memory deteriorated by maternal LPS exposure. Treadmill exercise increased cell proliferation and neurogenesis in the hippocampal dentate gyrus of the rats born of the LPS-exposed maternal rats. Treadmill exercise increased BDNF and TrkB expression in the hippocampus of the rats born of the LPS-exposed maternal rats. These effects of treadmill exercise were similarly appeared at both mild-intensity and moderate-intensity.
母体感染是导致新生儿脑损伤及后期发育障碍的重要因素。在本研究中，我们调查了跑步机运动强度对脂多糖（LPS）暴露的母鼠所产大鼠的短期记忆、海马神经发生以及脑源性神经营养因子（BDNF）和酪氨酸激酶受体B（TrkB）表达的影响。将大鼠分为六组：对照组、轻度运动组、中度运动组、母鼠LPS暴露组、母鼠LPS暴露及轻度运动组、母鼠LPS暴露及中度运动组。运动组的大鼠每周5次、每次30分钟在跑步机上强迫跑步，持续4周。运动负荷为轻度运动组以8米/分钟速度跑步，中度运动组以14米/分钟速度跑步。通过阶梯式回避任务中的潜伏期来测定短期记忆。进行5-溴-2'-脱氧尿苷免疫组化以确定海马细胞增殖和神经发生。进行蛋白质免疫印迹分析以检测BDNF和TrkB表达。在本研究中，跑步机运动改善了因母鼠LPS暴露而恶化的短期记忆。跑步机运动增加了LPS暴露母鼠所产大鼠海马齿状回中的细胞增殖和神经发生。跑步机运动增加了LPS暴露母鼠所产大鼠海马中BDNF和TrkB的表达。跑步机运动的这些作用在轻度和中度强度下均有类似表现。
